Department of Anesthesia and Pain Medicine, Pusan National University Yangsan Hospital, 20, Geumo-ro, Beomeo-ri, Mulgeumeup, Yangsan, 626-770, Republic of Korea.
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea.
J Anesth. 2018 Apr;32(2):204-210. doi: 10.1007/s00540-018-2465-0. Epub 2018 Feb 20.
Sevoflurane is commonly used in general anesthesia for premature neonates. The main mechanism of retinopathy of prematurity (ROP) is increased levels of vascular endothelial growth factor (VEGF). For the investigation of sevoflurane's effect on angiogenesis, the angiogenesis and VEGF expression in the retina were measured after administering sevoflurane in an oxygen-induced retinopathy mice model.
The mice were divided into the normoxic group (Nc and Ns group; n = 6) and the ROP group (C, Rc, and Rs group; n = 6). Rc group were exposed to 75% oxygen for 5 days beginning on postnatal day (P) 7, and then returned to room air. Age-matched mice in the C group were exposed to room air. To observe angiogenesis of the retina, the mice were sacrificed on P16. The Rs group was exposed to 2 vol% sevoflurane for 2 h on P12, P13, and P14 with 40% oxygen.
The angiogenic area and the spreading distance of vessels on P4 were statistically decreased in the Ns group, compared to the Nc group. The avascular area on P16 was significantly increased and the expression of VEGF was suppressed in the Rs group compared to the Rc group.
Sevoflurane can inhibit retinal angiogenesis via suppressing VEGF expression in an OIR mice model with exposure to relative hypoxia. Nevertheless, it is still difficult to apply the results of this study immediately to humans because of the heterogeneity of responses to sevoflurane.
七氟醚常用于早产儿的全身麻醉。早产儿视网膜病变（ROP）的主要发病机制是血管内皮生长因子（VEGF）水平升高。为了研究七氟醚对血管生成的影响，在氧诱导的视网膜病变小鼠模型中给予七氟醚后，测量了视网膜的血管生成和 VEGF 表达。
将小鼠分为常氧组（Nc 和 Ns 组；n=6）和 ROP 组（C、Rc 和 Rs 组；n=6）。Rc 组于生后第 7 天开始暴露于 75%氧气中 5 天，然后返回室内空气。C 组中年龄匹配的小鼠暴露于室内空气。为了观察视网膜的血管生成，于 P16 处死小鼠。Rs 组于 P12、P13 和 P14 时在 40%氧气中暴露于 2 体积%七氟醚 2 小时。
与 Nc 组相比，Ns 组 P4 时血管生成区和血管延伸距离统计学上降低。与 Rc 组相比，Rs 组 P16 时无血管区明显增加，VEGF 表达受抑制。
在相对低氧暴露的 OIR 小鼠模型中，七氟醚通过抑制 VEGF 表达抑制视网膜血管生成。然而，由于对七氟醚的反应存在异质性，因此很难立即将本研究的结果应用于人类。
