Ri Hyun-Su, Bae Sun Sik, Ha Jung Min, Kim Hee Young, Baek Seung-Hoon
Department of Anesthesia and Pain Medicine, Pusan National University Yangsan Hospital, Pusan National University School of Medicine, Geumo-ro 20, Yangsan, 50612, Republic of Korea.
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Busandaehak-ro 49, Mulgeum-eup, Yangsan, 50612, Republic of Korea.
J Anesth. 2020 Jun;34(3):352-357. doi: 10.1007/s00540-020-02752-4. Epub 2020 Feb 25.
Retinopathy of prematurity (ROP) is an ocular disorder that primarily occurs in premature infants and is the most common cause of vision impairment. This study examined the effect of desflurane on angiogenesis in a mouse model of oxygen-induced retinopathy (OIR).
Mice were randomly allocated to the control (C), ROP control (R), or ROP with desflurane exposure (R) group. To induce ROP, 7-day-old mice were exposed to 75% oxygen in a chamber for 5 days [postnatal days (P) 7-12], and thereafter returned to room air. Age-matched mice exposed to room air formed the C group. The R group was exposed to 8% desflurane for 2 h on P12, P13, and P14 with 40% oxygen. To observe changes in angiogenesis of the retina, mice were sacrificed at P16.
The ratio of avascular area/total retinal area was not changed significantly in the R group, compared to the R group. The expression of endothelial growth factor A (VEGF-A) and hypoxia inducible factor-1α (HIF-1α) in the R group and R group was not significantly different.
Desflurane does not have a significant influence on retinal angiogenesis via HIF-1α and VEGF-A expression in the OIR mouse model. However, these findings are not directly applicable to premature infants, and it is thus necessary to perform further studies to determine the effect of desflurane on angiogenesis.
早产儿视网膜病变（ROP）是一种主要发生于早产儿的眼部疾病，是视力损害的最常见原因。本研究在氧诱导视网膜病变（OIR）小鼠模型中检测了地氟醚对血管生成的影响。
将小鼠随机分为对照组（C）、ROP对照组（R）或地氟醚暴露ROP组（R）。为诱导ROP，7日龄小鼠在氧舱中暴露于75%氧气5天[出生后第（P）7 - 12天]，之后恢复至室内空气环境。暴露于室内空气的年龄匹配小鼠构成C组。R组在P12、P13和P14时于40%氧气环境下暴露于8%地氟醚2小时。为观察视网膜血管生成的变化，在P16处死小鼠。
与R组相比，R组无血管区/视网膜总面积的比例无显著变化。R组和R组中内皮生长因子A（VEGF - A）和缺氧诱导因子 - 1α（HIF - 1α）的表达无显著差异。
在地氟醚OIR小鼠模型中，地氟醚对通过HIF - 1α和VEGF - A表达的视网膜血管生成无显著影响。然而，这些发现不能直接应用于早产儿，因此有必要进行进一步研究以确定地氟醚对血管生成的影响。
