El Hamrani Dounia, Chepied Amandine, Même William, Mesnil Marc, Defamie Norah, Même Sandra
Centre de Biophysique Moléculaire, CNRS UPR4301, Rue Charles Sadron, CS 80054, 45071, Orléans Cedex 2, France.
Laboratoire Signalisation et Transports Ioniques Membranaires, University of Poitiers, ERL-CNRS 7368, 1 rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 09, France.
MAGMA. 2018 Aug;31(4):565-576. doi: 10.1007/s10334-018-0679-7. Epub 2018 Feb 20.
Using non-invasive magnetic resonance (MR) techniques and a histological approach, we assessed the outcomes of perinatal exposure at a low dose of 3,3'-DCBPA (2-chloro-4-[1-(3-chloro-4-hydroxyphenyl)-1-methylethyl]phenol) and/or 3,5-DCBPA (2,6-dichloro-4-[1-(4-hydroxyphenyl)-1-methylethyl]phenol) on mice livers.
Fertilized female Swiss mice were injected intraperitoneally during gestation and lactation with either vehicle control, 20 μg/kg/day of BPA, 3,5-DCBPA, 3,3'-DCBPA or a mixture (mix-DCBPA). Complementary methods were used to evaluate, in male and female pups, (1) liver structure by texture analysis of images obtained through MR imaging (MRI) and histology, (2) hepatic lipid composition through in vivo H MR spectroscopy (H MRS).
Principal component analysis of texture parameters showed no structural modification of the liver with BPA and DCBPA treatments. Accordingly, no hepatic microvesicular steatosis was observed through hematoxylin-eosin staining. Compared to control, MRS revealed no difference in lipid composition for BPA, 3,5-DCBPA or 3,3'-DCBPA groups. However, MRS detected a significant increase in the mix-DCBPA groups for the saturated component of fatty acids (FA), total unsaturated FA bond index and polyunsaturated FA bond index.
Prior to any structural changes, polyunsaturated fatty acids significantly increased in young male and female mice exposed perinatally at a low dose to a mixture of dichlorinated BPA.
我们使用非侵入性磁共振（MR）技术和组织学方法，评估低剂量3,3'-二氯双酚A（2-氯-4-[1-(3-氯-4-羟基苯基)-1-甲基乙基]苯酚）和/或3,5-二氯双酚A（2,6-二氯-4-[1-(4-羟基苯基)-1-甲基乙基]苯酚）围产期暴露对小鼠肝脏的影响。
在妊娠和哺乳期，给受精的雌性瑞士小鼠腹腔注射载体对照、20μg/kg/天的双酚A、3,5-二氯双酚A、3,3'-二氯双酚A或混合物（混合二氯双酚A）。采用补充方法评估雄性和雌性幼崽的：（1）通过磁共振成像（MRI）和组织学获得的图像纹理分析来评估肝脏结构；（2）通过体内氢磁共振波谱（H MRS）评估肝脏脂质组成。
纹理参数的主成分分析表明，双酚A和二氯双酚A处理后肝脏结构无改变。因此，苏木精-伊红染色未观察到肝脏微泡性脂肪变性。与对照组相比，MRS显示双酚A、3,5-二氯双酚A或3,3'-二氯双酚A组的脂质组成无差异。然而，MRS检测到混合二氯双酚A组脂肪酸（FA）的饱和成分、总不饱和FA键指数和多不饱和FA键指数显著增加。
在出现任何结构变化之前，围产期低剂量暴露于二氯双酚A混合物的年轻雄性和雌性小鼠体内多不饱和脂肪酸显著增加。
