Altamirano Gabriela A, Muñoz-de-Toro Mónica, Luque Enrique H, Gómez Ayelén L, Delconte Melisa B, Kass Laura
Instituto de Salud y Ambiente del Litoral (ISAL, CONICET-UNL), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Instituto de Salud y Ambiente del Litoral (ISAL, CONICET-UNL), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Mol Cell Endocrinol. 2015 Aug 15;411:258-67. doi: 10.1016/j.mce.2015.05.007. Epub 2015 May 11.
To evaluate whether bisphenol A (BPA) modifies the synthesis, composition and/or profile of fatty acids (FAs) in the mammary glands of perinatally exposed animals, pregnant rats were orally exposed to 0, 0.6 or 52 µg BPA/kg/day from gestation day (GD) 9 until weaning. F1 females were bred, and on GD21, lactation day 2 (LD2) and LD10, mammary glands were obtained. On LD10, milk samples were collected, and FA profiles and lipid compositions were established. On GD21 and LD2, BPA exposure delayed mammary alveolar maturation and modified the synthesis of milk fat globules. On LD10, mammary gland histo-architecture was restored; however, the milk of BPA-exposed F1 dams had a FA profile and lipid concentration different from those of the control milk. Furthermore, the body weight gain of BPA52 F2 pups was increased compared with control animals. Thus, perinatal exposure to BPA modifies milk quality, compromising the normal growth of offspring.
为评估双酚A（BPA）是否会改变围产期暴露动物乳腺中脂肪酸（FAs）的合成、组成和/或分布，将怀孕大鼠从妊娠第9天（GD）至断奶期间经口暴露于0、0.6或52µg BPA/kg/天。对F1代雌性大鼠进行繁殖，并在GD21、泌乳第2天（LD2）和LD10时获取乳腺。在LD10时，收集乳汁样本，并确定脂肪酸分布和脂质组成。在GD21和LD2时，BPA暴露延迟了乳腺腺泡成熟并改变了乳脂肪球的合成。在LD10时，乳腺组织结构得以恢复；然而，暴露于BPA的F1代母鼠的乳汁具有与对照乳汁不同的脂肪酸分布和脂质浓度。此外，与对照动物相比，BPA52组F2代幼崽的体重增加。因此，围产期暴露于BPA会改变乳汁质量，影响后代的正常生长。
