Stenholm Åke, Hedeland Mikael, Arvidsson Torbjörn, Pettersson Curt E
a Division of Analytical Pharmaceutical Chemistry, Uppsala University , Uppsala , Sweden.
b GE Healthcare Bio-Sciences AB , Uppsala , Sweden.
Environ Technol. 2019 Aug;40(19):2460-2472. doi: 10.1080/09593330.2018.1444098. Epub 2018 Mar 4.
This paper describes the search for procedures through which the xenobiotic pollutant diclofenac can be removed from non-sterile aquatic systems. Specifically, adsorption to solid supports (carriers) in combination with biodegradation by non-immobilized and immobilized white rot fungus were investigated. Batch experiments using polyurethane foam (PUF)-carriers resulted in 99.9% diclofenac removal after 4 h, with monolayer adsorption of diclofenac to carrier and glass surfaces accounting for most of the diclofenac decrease. Enzymatic reactions contributed less, accounting for approximately < 0.5% of this decrease. In bioreactor experiments using PUF-carriers, an initial 100% removal was achieved with biodegradation contributing approximately 7%. In batch experiments that utilized polyethylene-carriers with negligible immobilization of , a 98% total diclofenac removal was achieved after one week, with a biodegradation contribution of approximately 14%. Five novel enzyme-catalyzed biodegradation products were tentatively identified in the batch-wise and bioreactor experiments using full scan ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry. Both reduction and oxidation products were found, with the contents estimated to be at µg L concentration levels.
本文描述了寻找从非无菌水生系统中去除外源污染物双氯芬酸的方法。具体而言，研究了双氯芬酸在固体载体上的吸附作用以及非固定化和固定化白腐真菌的生物降解作用。使用聚氨酯泡沫（PUF）载体的批次实验在4小时后实现了99.9%的双氯芬酸去除率，双氯芬酸在载体和玻璃表面的单层吸附占双氯芬酸减少量的大部分。酶促反应贡献较小，约占减少量的<0.5%。在使用PUF载体的生物反应器实验中，最初实现了100%的去除率，生物降解贡献约为7%。在使用聚乙烯载体且固定化可忽略不计的批次实验中，一周后双氯芬酸的总去除率达到98%，生物降解贡献约为14%。在使用全扫描超高效液相色谱-四极杆/飞行时间质谱的批次实验和生物反应器实验中，初步鉴定出了五种新型酶催化生物降解产物。发现了还原产物和氧化产物，其含量估计处于μg L浓度水平。
