Liu Min-Jiao, Jin Chun-Zhi, Park Dong-Jin, Asem Mipeshwaree Devi, Xiao Min, Salam Nimaichand, Li Wen-Jun, Kim Chang-Jin
Industrial Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Department of Bio-Molecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1215-1219. doi: 10.1099/ijsem.0.002654. Epub 2018 Feb 21.
An aerobic actinobacterium, strain AN130378, was isolated from a soil sample collected in Korea and subjected to taxonomic investigation using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain AN130378 is a member of the genus Stackebrandtia, with sequence similarities of 97.3 % to Stackebrandtia albiflava YIM 45751 and 97.1 % to Stackebrandtia endophytica YIM 64602. The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose and xylose. The major menaquinones were MK-11(H4), MK-10(H4) and MK-11(H6), while the major fatty acids were identified as iso-C15 : 0, anteiso-C17 : 0, anteiso-C15 : 0, iso-C17 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids. The G+C content of the genomic DNA was determined to be 67.7 mol%. All chemotaxonomic and genotypic data indicated that the strain belongs to the genus Stackebrandtia. On the basis of morphological, chemotaxonomic data and phylogenetic analysis, strain AN130378 is considered to represent a novel species within the genus Stackebrandtia, for which the name Stackebrandtia soli sp. nov. is proposed. The type strain is AN130378 (=DSM 103573=KCTC 39809).
从从从韩国采集的土壤样本中分离出一株好氧放线菌AN130378，并采用多相分类法对其进行分类学研究。基于16S rRNA基因序列数据的系统发育分析表明，菌株AN130378是Stackebrandtia属的成员，与白黄Stackebrandtia albiflava YIM 45751的序列相似性为97.3%，与内生Stackebrandtia endophytica YIM 64602的序列相似性为97.1%。全细胞水解产物含有内消旋二氨基庚二酸作为诊断性二氨基酸，以及半乳糖、葡萄糖和木糖。主要甲基萘醌为MK-11(H4)、MK-10(H4)和MK-11(H6)；主要脂肪酸鉴定为异-C15 : 0、anteiso-C17 : 0、anteiso-C15 : 0、异-C17 : 0和特征性总和3（C16 : 1ω6c和/或C16 : 1ω7c）。极性脂质鉴定为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰甲基乙醇胺、一种未鉴定的氨基磷脂、四种未鉴定的糖脂、三种未鉴定的磷脂和两种未鉴定的极性脂质。基因组DNA的G+C含量测定为67.7mol%。所有化学分类和基因型数据表明该菌株属于Stackebrandtia属。基于形态学、化学分类数据和系统发育分析，菌株AN130378被认为代表Stackebrandtia属内的一个新物种，为此提出名称Stackebrandtia soli sp. nov.。模式菌株为AN130378（=DSM 103573=KCTC 39809）。
