Terena Stella Maris Lins, Fernandes Kristianne Porta Santos, Bussadori Sandra Kalil, Brugnera Junior Aldo, de Fátima Teixeira da Silva Daniela, Magalhães Eric Moreno Ramos, Ferrari Raquel Agnelli Mesquita
1 Posgraduate Program, Department of Biophotonics Applied to Health Sciences, Universidade Nove de Julho-UNINOVE , São Paulo, Brazil .
2 Posgraduate Program, Department of Rehabilitation Sciences and Biophotonics Applied to Health Sciences, Universidade Nove de Julho-UNINOVE , São Paulo, Brazil .
Photomed Laser Surg. 2018 Mar;36(3):130-136. doi: 10.1089/pho.2017.4302. Epub 2018 Feb 21.
The photobiomodulation using the low-level laser therapy (LLLT) exerts a positive modulating effect on the synthesis of collagen in skeletal muscles and tendons. However, few studies have addressed this effect during the compensatory overload.
Evaluate the effect of infrared laser on the deposition and organization of collagen fibers in muscle and tendon tissue during compensatory overload of the plantar muscle in rats.
Wistar rats were submitted to bilateral ablation of the synergist muscles of the hind paws and divided in groups: Control, Hypertrophy, and Hypertrophy (H)+LLLT (780 nm, 40 mW, 9.6 J/cm and 10 s/point, 8 points, total energy 3.2 J, daily), evaluated at 7 and 14 days. Muscle cuts were stained with Picrosirius-Red and hematoxylin-eosin and tendon cuts were submitted to birefringence for determination of collagen distribution and organization.
After 7 days an increase was observed in the area between beam muscles in H+LLLT (25.45% ± 2.56) in comparison to H (20.3% ± 3.31), in mature fibers and fibrilis in H+LLLT (29346.88 μm ± 2182.56; 47602.8 μm ± 2201.86 respectively) in comparison to H (26656.5 μm ± 1880.46; 45630.34 μm ± 2805.82 respectively) and in the collagen area in H+LLLT (2.25% ± 0.19) in comparison to H (2.0% ± 0.15). However, after 14 days a reduction was observed in the area between beam muscles in H+LLLT (13.88% ± 2.54) in comparison to H (19.1% ± 2.61), in fibrils and mature fibers in H+LLLT (17174.1 μm ± 2563.82; 32634.04 μm ± 1689.38 respectively) in comparison to H (55249.86 μm ± 1992.65; 44318.36 μm ± 1759.57) and in the collagen area in H+LLLT (1.76% ± 0.16) in comparison to H (2.09 ± 0.27). A greater organization of collagen fibers in the tendon was observed after 7 and 14 days in H+LLLT groups.
Infrared laser irradiation induces an improvement in collagen organization in tendons and a reduction in the total area of collagen in muscles during compensatory atrophy following the ablation of synergist muscles.
使用低强度激光疗法（LLLT）进行的光生物调节对骨骼肌和肌腱中的胶原蛋白合成具有积极的调节作用。然而，很少有研究探讨代偿性超负荷期间的这种作用。
评估红外激光对大鼠足底肌肉代偿性超负荷期间肌肉和肌腱组织中胶原纤维沉积和排列的影响。
将Wistar大鼠双侧后爪协同肌切除，并分为对照组、肥大组和肥大（H）+LLLT组（780 nm，40 mW，9.6 J/cm且每点10 s，共8点，总能量3.2 J，每日），分别在7天和14天时进行评估。肌肉切片用天狼星红-苏木精-伊红染色，肌腱切片进行双折射以确定胶原蛋白的分布和排列。
7天后，与肥大组（20.3%±3.31）相比，H+LLLT组（25.45%±2.56）束间肌区域增加；与肥大组（分别为26656.5μm±1880.46；45630.34μm±2805.82）相比，H+LLLT组成熟纤维和原纤维（分别为29346.88μm±2182.56；47602.8μm±2201.86）增加；与肥大组（2.0%±0.15）相比，H+LLLT组胶原蛋白区域（2.25%±0.19）增加。然而，14天后，与肥大组（19.1%±2.61）相比，H+LLLT组束间肌区域减少（13.88%±2.54）；与肥大组（分别为55249.86μm±1992.65；44318.36μm±1759.57）相比，H+LLLT组原纤维和成熟纤维（分别为17174.1μm±2563.82；32634.04μm±1689.38）减少；与肥大组（2.09±0.27）相比，H+LLLT组胶原蛋白区域（1.76%±0.16）减少。在7天和14天时，H+LLLT组肌腱中胶原纤维的排列更有序。
在协同肌切除后的代偿性萎缩过程中，红外激光照射可改善肌腱中胶原纤维的排列，并减少肌肉中胶原蛋白的总面积。
