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体外研究低水平激光治疗对早期韧带成纤维细胞愈合过程中 I 型胶原合成的影响。

In-vitro study on type I collagen synthesis in low-level laser therapy on the early ligament fibroblasts' healing process.

机构信息

Rehabilitation Science Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Colombia.

Neuroscience and Mental Health Institute and Faculty of Rehabilitation Medicine, University of Alberta, Edmonton, Alberta, Canada.

出版信息

Lasers Med Sci. 2024 Aug 29;39(1):225. doi: 10.1007/s10103-024-04151-7.

DOI:10.1007/s10103-024-04151-7
PMID:39207591
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11362177/
Abstract

BACKGROUND

Low-level Laser Therapy (LLLT) has demonstrated its potential in promoting fiber matrix maturation, collagen synthesis, and fibroblast proliferation, contributing to tissue regeneration. Our study aimed to investigate the impact of LLLT on collagen type I synthesis, cell proliferation, and viability in human ligament fibroblasts derived from the Anterior Cruciate Ligament (ACL).

METHODS

Tissue samples were obtained from individuals undergoing arthroscopic ACL reconstruction surgery. Primary human fibroblasts were isolated, and immunohistochemical assays confirmed their characteristics. LLLT at 850 nm was administered in three groups: Low dose (1.0 J/cm²), High dose (5.0 J/cm²), and Control (0.0 J/cm²). Cell viability was calculated using a membrane integrity assay, proliferation was determined by automated counting, and collagen type I concentration in cell culture was measured using an immunoassay.

RESULTS

Fibroblasts showed decreased viability after low and high doses of LLLT, increased proliferation at the low dose, and increased collagen synthesis at the high dose on day 10 for both sexes after treatment.

CONCLUSION

Our study demonstrated that LLLT may improve the early ligament healing process by increasing cell proliferation at the low dose and enhancing collagen type I synthesis at the high dose in human ligament fibroblasts.

摘要

背景

低水平激光疗法（LLLT）已证明其在促进纤维基质成熟、胶原蛋白合成和纤维母细胞增殖方面的潜力，有助于组织再生。我们的研究旨在探讨 LLLT 对源自前交叉韧带（ACL）的人韧带成纤维细胞中 I 型胶原蛋白合成、细胞增殖和活力的影响。

方法

从接受关节镜 ACL 重建手术的个体中获取组织样本。分离原代人成纤维细胞，并通过免疫组织化学检测确认其特征。850nm 的 LLLT 在三组中进行：低剂量（1.0J/cm²）、高剂量（5.0J/cm²）和对照组（0.0J/cm²）。使用膜完整性测定法计算细胞活力，通过自动计数确定增殖，使用免疫测定法测量细胞培养物中 I 型胶原蛋白的浓度。

结果

低剂量和高剂量的 LLLT 后，成纤维细胞的活力下降，低剂量时增殖增加，治疗后第 10 天，两性的 I 型胶原蛋白合成增加。

结论

我们的研究表明，LLLT 可以通过在低剂量时增加细胞增殖，在高剂量时增强 I 型胶原蛋白合成，从而改善人韧带成纤维细胞的早期韧带愈合过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/617e00f18585/10103_2024_4151_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/62da1a35a385/10103_2024_4151_Figb_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/99f053fe71a3/10103_2024_4151_Figa_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/b03600caa11e/10103_2024_4151_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/dccf1775aeb1/10103_2024_4151_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/617e00f18585/10103_2024_4151_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/62da1a35a385/10103_2024_4151_Figb_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/99f053fe71a3/10103_2024_4151_Figa_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/b03600caa11e/10103_2024_4151_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/dccf1775aeb1/10103_2024_4151_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d886/11362177/617e00f18585/10103_2024_4151_Fig5_HTML.jpg

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