Sheykhbaglou Roghayyeh, Sedghi Mohammad, Fathi-Achachlouie Bahram
Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Daneshgah Street, 56199-11367, Ardabil, Iran.
Department of Food Science and Technology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Daneshgah Street, 56199-11367, Ardabil, Iran.
An Acad Bras Cienc. 2018 Jan-Mar;90(1):485-494. doi: 10.1590/0001-3765201820160251.
Soybean (Glycine max L.) seed contains amounts of protein, lipid, carbohydrate and mineral elements, which protein and lipid have been known as a main part for soybean's trade value. In this study, in order to investigate the effect of ferrous nano-oxide particles on nutritional compounds of soybean seed, an experiment with 5 treatments and 3 replications was conducted as a randomized complete block design. Treatments were 5 concentrations of ferrous nano-oxide particles including 0, 0.25, 0.5, 0.75 and 1 g L-1 which were sprayed 3 times at 4 and 8 leaves stage and pod initiation. Lipid and protein contents, fatty acids profile, some of mineral elements such as Fe, Mg, Ca and P, chlorophyll a, b and total chlorophyll content were determined. Results showed that solution containing ferrous nano-oxide particles had significant effect on nutritional compounds of soybean seed (P<0.01) compared to control. The highest content of lipid and protein (25.4 and %33.8, respectively) observed by applying 0.75 g L-1 of ferrous nano-oxide and the lowest content was also in control. Changes in the trends of fatty acids profile (palmitic, oleic, linoleic and linolenic acids), some of mineral elements (Fe, Mg, Ca and P) and chlorophyll contents were similar to lipid and protein levels which by increasing in concentration of ferrous nano-oxide from 0 to 0.75 g L-1 all measured parameters also increased, but reduction in all parameters was observed in concentration from 0.75 to 1 g L-1. In conclusion, application of 0.75 to 1 g L-1 ferrous nano-oxide had the best effect on the nutrient composition of soybean seed.
大豆（Glycine max L.）种子含有一定量的蛋白质、脂质、碳水化合物和矿物质元素，其中蛋白质和脂质是大豆商业价值的主要组成部分。在本研究中，为了探究纳米氧化亚铁颗粒对大豆种子营养成分的影响，以随机完全区组设计进行了一项包含5个处理、3次重复的实验。处理为5种纳米氧化亚铁颗粒浓度，分别为0、0.25、0.5、0.75和1 g L-1，在4叶期、8叶期和结荚初期各喷施3次。测定了脂质和蛋白质含量、脂肪酸谱、一些矿物质元素（如铁、镁、钙和磷）、叶绿素a、叶绿素b和总叶绿素含量。结果表明，与对照相比，含有纳米氧化亚铁颗粒的溶液对大豆种子的营养成分有显著影响（P<0.01）。施用0.75 g L-1纳米氧化亚铁时观察到脂质和蛋白质含量最高（分别为25.4%和33.8%），对照中的含量最低。脂肪酸谱（棕榈酸、油酸、亚油酸和亚麻酸）、一些矿物质元素（铁、镁、钙和磷）和叶绿素含量的变化趋势与脂质和蛋白质水平相似，随着纳米氧化亚铁浓度从0增加到0.75 g L-1，所有测定参数均增加，但在浓度从0.75 g L-1增加到1 g L-1时，所有参数均下降。总之，施用0.75至1 g L-1纳米氧化亚铁对大豆种子的营养成分影响最佳。
