Shukla Madhulika, Tabassum Rizwana, Singh Rajendra, Dhar Dolly Wattal
Indian J Exp Biol. 2016 Jul;54(7):482-487.
Effects of the environmental variables such as light intensity (Vmol photons m⁻² S⁻¹), temperature (C) and CO₂ concentration (ppm) on chlorophyll, total soluble proteins and lipids were studied in selected microalgal strains from Chlorophyceae (Chlamydomonas sp., Scenedesmus sp., Chlorella sp., Kirchneriella sp.) and cyanobacteria (Nostoc sp.1, Anabaena sp., Nostoc sp. 2, Cylindrospermum sp.). Cultures were grown under controlled conditions at the National Phytotron Facility, Indian Agricultural Research Institute (IARI), New Delhi. Our results showed that chlorophyll concentration enhanced with increased C0₂. Chlorella exhibited the highest chlorophyll at 850 ppm CO₂ and 28C; for Chlamydomonas it was at 78 μmol photons m⁻² S⁻¹ light intensity. In Cylindrospernum, total soluble proteins decreased with enhanced C0₂, and were highest at 18C. In Anabaena, a light intensity of 65 μmol photons m⁻² S⁻¹ was best for maximum total soluble proteins. In Chlorella, CO₂ @ 850 ppm was most suited for maximum lipid accumulation. In Kirchneriella, increase in temperature, from 18C up to 370C, increased total lipids; the highest was at 28'C. In Chlamydomonas, the light intensity of 78 μmol photons m⁻² S⁻¹ was optimum for lipid accumulation and the maximum total lipids was 30.8 (% dry wt.).
研究了光强（微摩尔光子·平方米⁻²·秒⁻¹）、温度（℃）和二氧化碳浓度（ppm）等环境变量对绿藻门（衣藻属、栅藻属、小球藻属、基氏藻属）和蓝细菌（念珠藻属1、鱼腥藻属、念珠藻属2、柱孢藻属）中选定微藻菌株叶绿素、总可溶性蛋白和脂质的影响。培养物在新德里印度农业研究机构（IARI）的国家植物培养设施的可控条件下生长。我们的结果表明，叶绿素浓度随二氧化碳增加而提高。小球藻在850 ppm二氧化碳和28℃时叶绿素含量最高；对于衣藻来说，在78微摩尔光子·平方米⁻²·秒⁻¹光强下叶绿素含量最高。在柱孢藻中，总可溶性蛋白随二氧化碳增加而降低，在18℃时最高。在鱼腥藻中，65微摩尔光子·平方米⁻²·秒⁻¹的光强最有利于总可溶性蛋白达到最大值。在小球藻中，850 ppm的二氧化碳最适合脂质积累达到最大值。在基氏藻中，温度从18℃升高到37℃，总脂质增加；在28℃时最高。在衣藻中，78微摩尔光子·平方米⁻²·秒⁻¹的光强最适合脂质积累，最大总脂质含量为30.8（%干重）。
