Department of Environmental Engineering and Pioneer Research Center for Controlling of HAB, Chosun University, Gwangju, 501-759, South Korea,
Bioprocess Biosyst Eng. 2014 Feb;37(2):99-106. doi: 10.1007/s00449-013-0920-8. Epub 2013 May 3.
The growth and total lipid content of four green microalgae (Chlorella sp., Chlorella vulgaris CCAP211/11B, Botryococcus braunii FC124 and Scenedesmus obliquus R8) were investigated under different culture conditions. Among the various carbon sources tested, glucose produced the largest biomass or microalgae grown heterotrophically. It was found that 1% (w/v) glucose was actively utilized by Chlorella sp., C. vulgaris CCAP211/11B and B. braunii FC124, whereas S. obliquus R8 preferred 2% (w/v) glucose. No significant difference in biomass production was noted between heterotrophic and mixotrophic (heterotrophic with light illumination/exposure) growth conditions, however, less production was observed for autotrophic cultivation. Total lipid content in cells increased by approximately two-fold under mixotrophic cultivation with respect to heterotrophic and autotrophic cultivation. In addition, light intensity had an impact on microalgal growth and total lipid content. The highest total lipid content was observed at 100 μmol m(-2)s(-1) for Chlorella sp. (22.5%) and S. obliquus R8 (23.7%) and 80 μmol m(-2)s(-1) for C. vulgaris CCAP211/11B (20.1%) and B. braunii FC124 (34.9%).
研究了四种绿色微藻（Chlorella sp.、Chlorella vulgaris CCAP211/11B、Botryococcus braunii FC124 和 Scenedesmus obliquus R8）在不同培养条件下的生长和总脂质含量。在测试的各种碳源中，葡萄糖产生最大的生物量或异养生长的微藻。研究发现，1%（w/v）葡萄糖被 Chlorella sp.、C. vulgaris CCAP211/11B 和 B. braunii FC124 积极利用，而 S. obliquus R8 则更喜欢 2%（w/v）葡萄糖。异养和混合营养（异养光照/暴露）生长条件下的生物量生产没有显著差异，但自养培养的产量较低。与异养和自养培养相比，混合营养培养下细胞的总脂质含量增加了约两倍。此外，光照强度对微藻的生长和总脂质含量有影响。Chlorella sp.（22.5%）和 S. obliquus R8（23.7%）的总脂质含量最高，为 100 μmol m(-2)s(-1)，C. vulgaris CCAP211/11B（20.1%）和 B. braunii FC124（34.9%）的总脂质含量最高，为 80 μmol m(-2)s(-1)。
