纤连蛋白糖基化调节成纤维细胞的黏附与铺展。
Fibronectin glycosylation modulates fibroblast adhesion and spreading.
作者信息
Jones G E, Arumugham R G, Tanzer M L
出版信息
J Cell Biol. 1986 Nov;103(5):1663-70. doi: 10.1083/jcb.103.5.1663.
Abstract
The role of the carbohydrate residues of fibronectin concerning the specificities of that glycoprotein to interact with fibroblastic cell surfaces, gelatin, and heparin was examined. Tunicamycin was used to produce carbohydrate-depleted fibronectin; it was synthesized by cultured fibroblasts. Unglycosylated and glycosylated fibronectins were analyzed for their ability to bind gelatin and heparin, using affinity columns. Fibronectin-coated surfaces were used to quantitatively measure cell adhesion and spreading. The results showed that the lack of carbohydrates significantly increased the interaction of the protein with gelatin and markedly enhanced its ability to promote adhesion and spreading of fibroblasts. In contrast, the binding of fibronectin to heparin was not influenced by glycosylation. The composite data indicate that the Asn-linked oligosaccharides of fibronectin act as modulators of biological functions of the glycoprotein.
摘要
研究了纤连蛋白的碳水化合物残基在该糖蛋白与成纤维细胞表面、明胶和肝素相互作用特异性方面的作用。衣霉素用于产生碳水化合物缺失的纤连蛋白；它由培养的成纤维细胞合成。使用亲和柱分析了未糖基化和糖基化的纤连蛋白结合明胶和肝素的能力。用纤连蛋白包被的表面定量测量细胞黏附和铺展。结果表明，碳水化合物的缺失显著增加了该蛋白与明胶的相互作用，并显著增强了其促进成纤维细胞黏附和铺展的能力。相比之下，纤连蛋白与肝素的结合不受糖基化的影响。综合数据表明，纤连蛋白的天冬酰胺连接的寡糖作为该糖蛋白生物学功能的调节剂。
相似文献
1
Fibronectin glycosylation modulates fibroblast adhesion and spreading.纤连蛋白糖基化调节成纤维细胞的黏附与铺展。
J Cell Biol. 1986 Nov;103(5):1663-70. doi: 10.1083/jcb.103.5.1663.
2
Acylation of gelatin-agarose and the enhancement by heparin of fibronectin binding.明胶-琼脂糖的酰化作用以及肝素对纤连蛋白结合的增强作用。
Arch Biochem Biophys. 1988 Oct;266(1):181-8. doi: 10.1016/0003-9861(88)90248-2.
3
Interaction of the 70,000-mol-wt amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts.纤连蛋白70,000道尔顿氨基末端片段与成纤维细胞基质组装受体的相互作用。
J Cell Biol. 1985 Feb;100(2):364-74. doi: 10.1083/jcb.100.2.364.
4
Fibronectin-plasma membrane interaction in the adhesion of hemopoietic cells.纤连蛋白-质膜相互作用在造血细胞黏附中的作用
J Cell Biol. 1986 Aug;103(2):429-37. doi: 10.1083/jcb.103.2.429.
5
Interaction of fibronectin with its receptor on platelets.纤连蛋白与其在血小板上的受体之间的相互作用。
Cell. 1985 Sep;42(2):439-48. doi: 10.1016/0092-8674(85)90101-1.
6
Role of the heparin binding domain of fibronectin in attachment and spreading of human bone-derived cells.纤连蛋白的肝素结合结构域在人骨源细胞黏附与铺展中的作用
J Cell Sci. 1995 May;108 ( Pt 5):2083-92. doi: 10.1242/jcs.108.5.2083.
7
Myonexin: an 80-kDa glycoprotein that binds fibronectin and is located at embryonic myotendinous junctions.肌联蛋白：一种80千道尔顿的糖蛋白，可结合纤连蛋白，位于胚胎肌-腱连接处。
Dev Biol. 1990 Nov;142(1):103-14. doi: 10.1016/0012-1606(90)90154-b.
8
Inhibition of fibronectin binding to matrix components by nonenzymatic glycosylation.非酶糖基化对纤连蛋白与基质成分结合的抑制作用。
Diabetes. 1984 Oct;33(10):970-4. doi: 10.2337/diab.33.10.970.
9
Further characterization of the binding of fibronectin to gelatin reveals the presence of different binding interactions.纤连蛋白与明胶结合的进一步特征分析揭示了不同结合相互作用的存在。
Arch Biochem Biophys. 1993 Jul;304(1):181-8. doi: 10.1006/abbi.1993.1337.
10
Amino acid sequence of HSP-1, a major protein of stallion seminal plasma: effect of glycosylation on its heparin- and gelatin-binding capabilities.雄性马精液血浆主要蛋白质HSP-1的氨基酸序列：糖基化对其肝素结合和明胶结合能力的影响
Biochem J. 1995 Sep 1;310 ( Pt 2)(Pt 2):615-22. doi: 10.1042/bj3100615.
引用本文的文献
1
JMJD6 Dysfunction Due to Iron Deficiency in Preeclampsia Disrupts Fibronectin Homeostasis Resulting in Diminished Trophoblast Migration.子痫前期中缺铁导致的JMJD6功能障碍破坏纤连蛋白稳态，导致滋养层细胞迁移减少。
Front Cell Dev Biol. 2021 May 12;9:652607. doi: 10.3389/fcell.2021.652607. eCollection 2021.
2
Serum Glycoproteomic Alterations in Patients with Diabetic Retinopathy.糖尿病视网膜病变患者血清糖蛋白质组学改变
Proteomes. 2020 Sep 13;8(3):25. doi: 10.3390/proteomes8030025.
3
Identification of biomarkers associated with partial epithelial to mesenchymal transition in the secretome of slug over-expressing hepatocellular carcinoma cells.鉴定与过表达肝癌细胞中伪足分泌组中部分上皮间质转化相关的生物标志物。
Cell Oncol (Dordr). 2018 Aug;41(4):439-453. doi: 10.1007/s13402-018-0384-6. Epub 2018 Jun 1.
4
Cell adhesion to extracellular matrix is different in marine hydrozoans compared with vertebrates.与脊椎动物相比，海洋水螅虫纲动物中细胞与细胞外基质的黏附情况有所不同。
Rouxs Arch Dev Biol. 1995 Aug;204(7-8):465-476. doi: 10.1007/BF00360854.
5
Tenascin-C: Form versus function.腱生蛋白-C：形式与功能
Cell Adh Migr. 2015;9(1-2):48-82. doi: 10.4161/19336918.2014.987587.
6
Fabrication of functional fibronectin patterns by nanosecond excimer laser direct write for tissue engineering applications.采用纳秒准分子激光直写技术制作功能性纤维连接蛋白图案用于组织工程应用。
J Mater Sci Mater Med. 2013 Jul;24(7):1809-21. doi: 10.1007/s10856-013-4927-0. Epub 2013 Apr 25.
7
Fibronectin: functional character and role in alcoholic liver disease.纤维连接蛋白：功能特性及其在酒精性肝病中的作用。
World J Gastroenterol. 2011 May 28;17(20):2482-99. doi: 10.3748/wjg.v17.i20.2482.
8
Molecular heterogeneity of gelatin-binding proteins from human seminal plasma.人精液中胶凝蛋白结合蛋白的分子异质性。
Asian J Androl. 2010 May;12(3):363-75. doi: 10.1038/aja.2009.98. Epub 2010 Feb 22.
9
Myocilin promotes substrate adhesion, spreading and formation of focal contacts in podocytes and mesangial cells.肌纤蛋白促进足细胞和系膜细胞的底物黏附、铺展及黏着斑的形成。
Histochem Cell Biol. 2009 Feb;131(2):167-80. doi: 10.1007/s00418-008-0518-4. Epub 2008 Oct 15.
10
High-molecular-weight fibronectin synthesized by adenoid cystic carcinoma cells of salivary gland origin.唾液腺来源的腺样囊性癌细胞合成的高分子量纤连蛋白。
Jpn J Cancer Res. 1999 Mar;90(3):308-19. doi: 10.1111/j.1349-7006.1999.tb00749.x.
本文引用的文献
1
Analysis of skin fibroblast aggregation in Duchenne muscular dystrophy.杜氏肌营养不良症中皮肤成纤维细胞聚集的分析
J Cell Sci. 1981 Apr;48:291-300. doi: 10.1242/jcs.48.1.291.
2
The phosphate content of human fibronectin.人纤连蛋白的磷酸盐含量。
J Biol Chem. 1982 Apr 10;257(7):3890-5.
3
Domain structure of the carboxyl-terminal half of human plasma fibronectin.人血浆纤连蛋白羧基末端一半的结构域结构
J Biol Chem. 1983 Mar 10;258(5):3332-40.
4
Cell surface molecules and fibronectin-mediated cell adhesion: effect of proteolytic digestion of membrane proteins.细胞表面分子与纤连蛋白介导的细胞黏附：膜蛋白的蛋白水解消化作用
J Cell Biol. 1982 Jul;94(1):179-86. doi: 10.1083/jcb.94.1.179.
5
Dualistic nature of adhesive protein function: fibronectin and its biologically active peptide fragments can autoinhibit fibronectin function.黏附蛋白功能的二元性：纤连蛋白及其生物活性肽片段可自动抑制纤连蛋白功能。
J Cell Biol. 1984 Jul;99(1 Pt 1):29-36. doi: 10.1083/jcb.99.1.29.
6
Monensin inhibits initial spreading of cultured human fibroblasts.莫能菌素抑制培养的人成纤维细胞的初始铺展。
Nature. 1983;305(5932):315-7. doi: 10.1038/305315a0.
7
Monensin-induced inhibition of cell spreading in normal and dystrophic human fibroblasts.莫能菌素对正常和营养不良型人成纤维细胞细胞铺展的抑制作用。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4960-4. doi: 10.1073/pnas.81.15.4960.
8
Human placental (fetal) fibronectin: increased glycosylation and higher protease resistance than plasma fibronectin. Presence of polylactosamine glycopeptides and properties of a 44-kilodalton chymotryptic collagen-binding domain: difference from human plasma fibronectin.人胎盘（胎儿）纤连蛋白：糖基化增加，且比血浆纤连蛋白具有更高的蛋白酶抗性。存在多乳糖胺糖肽以及44千道尔顿胰凝乳蛋白酶敏感的胶原结合结构域的特性：与人血浆纤连蛋白的差异。
J Biol Chem. 1984 Mar 25;259(6):3962-70.
9
10
Similarities and differences between the fibronectins of normal and transformed hamster cells.正常仓鼠细胞和转化仓鼠细胞纤连蛋白之间的异同
J Biol Chem. 1981 Nov 25;256(22):11708-15.
Zotero 插件