Pizzey J, Witkowski J, Jones G
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4960-4. doi: 10.1073/pnas.81.15.4960.
Cultured skin fibroblasts from normal individuals and from patients with Duchenne muscular dystrophy spread equally rapidly when seeded on a glass substratum. Exposure to the ionophore monensin substantially suppresses normal and dystrophic fibroblast spreading in serum-free media for up to at least 100 min. Preincubation of normal fibroblasts with monensin causes a further reduction in cell spreading. Dystrophic fibroblasts fail to spread as well as normal cells after monensin preincubation. Such findings indicate that there is a delay in the secretion of functional adhesive surface proteins in monensin-preincubated normal fibroblasts and that this lag period is significantly longer in dystrophic fibroblasts. These data are consistent with findings of altered adhesive and secretory properties of fibroblasts from patients with Duchenne muscular dystrophy.
来自正常个体和杜兴氏肌肉营养不良症患者的培养皮肤成纤维细胞，接种在玻璃基质上时，其铺展速度相同。在无血清培养基中，暴露于离子载体莫能菌素会显著抑制正常和营养不良的成纤维细胞铺展，至少持续100分钟。用莫能菌素预孵育正常成纤维细胞会导致细胞铺展进一步减少。莫能菌素预孵育后，营养不良的成纤维细胞铺展能力不如正常细胞。这些发现表明，经莫能菌素预孵育的正常成纤维细胞中功能性粘附表面蛋白的分泌存在延迟，且在营养不良的成纤维细胞中，这个延迟期明显更长。这些数据与杜兴氏肌肉营养不良症患者的成纤维细胞粘附和分泌特性改变的研究结果一致。
