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番茄 MADS 框蛋白 SlCMB1 调控果实成熟过程中的乙烯生物合成和类胡萝卜素积累。

A tomato MADS-box protein, SlCMB1, regulates ethylene biosynthesis and carotenoid accumulation during fruit ripening.

机构信息

Laboratory of molecular biology of tomato, Bioengineering College, Chongqing University, Chongqing, People's Republic of China.

出版信息

Sci Rep. 2018 Feb 21;8(1):3413. doi: 10.1038/s41598-018-21672-8.

DOI:10.1038/s41598-018-21672-8
PMID:29467500
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5821886/
Abstract

The MADS-box transcription factors play essential roles in many physiological and biochemical processes of plants, especially in fruit ripening. Here, a tomato MADS-box gene, SlCMB1, was isolated. SlCMB1 expression declined with the fruit ripening from immature green to B + 7 (7 days after Breaker) fruits in the wild type (WT) and was lower in Nr and rin mutants fruits. Tomato plants with reduced SlCMB1 mRNA displayed delayed fruit ripening, reduced ethylene production and carotenoid accumulation. The ethylene production in SlCMB1-RNAi fruits decreased by approximately 50% as compared to WT. The transcripts of ethylene biosynthesis genes (ACS2, ACS4, ACO1 and ACO3), ethylene-responsive genes (E4, E8 and ERF1) and fruit ripening-related genes (RIN, TAGL1, FUL1, FUL2, LoxC and PE) were inhibited in SlCMB1-RNAi fruits. The carotenoid accumulation was decreased and two carotenoid synthesis-related genes (PSY1 and PDS) were down-regulated while three lycopene cyclase genes (CYCB, LCYB and LCYE) were up-regulated in transgenic fruits. Furthermore, yeast two-hybrid assay showed that SlCMB1 could interact with SlMADS-RIN, SlMADS1, SlAP2a and TAGL1, respectively. Collectively, these results indicate that SlCMB1 is a new component to the current model of regulatory network that regulates ethylene biosynthesis and carotenoid accumulation during fruit ripening.

摘要

MADS 框转录因子在植物的许多生理和生化过程中发挥着重要作用，尤其是在果实成熟过程中。在这里，分离得到了一个番茄 MADS 框基因 SlCMB1。在野生型（WT）和 Nr 和 rin 突变体果实中，SlCMB1 的表达随着果实从未成熟的绿色到 B + 7（破色后 7 天）的成熟过程而下降。SlCMB1 mRNA 减少的番茄植株表现出果实成熟延迟、乙烯生成和类胡萝卜素积累减少。与 WT 相比，SlCMB1-RNAi 果实中的乙烯生成减少了约 50%。乙烯生物合成基因（ACS2、ACS4、ACO1 和 ACO3）、乙烯响应基因（E4、E8 和 ERF1）和果实成熟相关基因（RIN、TAGL1、FUL1、FUL2、LoxC 和 PE）的转录物在 SlCMB1-RNAi 果实中受到抑制。类胡萝卜素积累减少，两个类胡萝卜素合成相关基因（PSY1 和 PDS）下调，而三个番茄红素环化酶基因（CYCB、LCYB 和 LCYE）上调。此外，酵母双杂交试验表明，SlCMB1 分别与 SlMADS-RIN、SlMADS1、SlAP2a 和 TAGL1 相互作用。综上所述，这些结果表明 SlCMB1 是调控果实成熟过程中乙烯生物合成和类胡萝卜素积累的调控网络的一个新组成部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/a990b18e2f9a/41598_2018_21672_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/a717b17a08ef/41598_2018_21672_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/496a14c8c954/41598_2018_21672_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/3c66d75f1c84/41598_2018_21672_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/3c548a78b20d/41598_2018_21672_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/b8d08be1d09f/41598_2018_21672_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/96d0ffd0d7c3/41598_2018_21672_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/a990b18e2f9a/41598_2018_21672_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/a717b17a08ef/41598_2018_21672_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/496a14c8c954/41598_2018_21672_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/3c66d75f1c84/41598_2018_21672_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/3c548a78b20d/41598_2018_21672_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/b8d08be1d09f/41598_2018_21672_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/96d0ffd0d7c3/41598_2018_21672_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8379/5821886/a990b18e2f9a/41598_2018_21672_Fig7_HTML.jpg

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