Ren Wenqing, Wang Han, Bai Jinjuan, Wu Feijie, He Yuke
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry, Jiangsu Second Normal University, Nanjing, China.
Front Plant Sci. 2018 Feb 6;9:73. doi: 10.3389/fpls.2018.00073. eCollection 2018.
Many vegetable crops of are characterized by their typical types of leaf curvature. Leaf curvature in the right direction and to the proper degree is important for the yield and quality of green vegetable products, when cultivated under stress conditions. Recent research has unveiled some of the roles of miRNAs in crops such as how they regulate the timing of leafy head initiation and shape of the leafy head. However, the molecular mechanism underlying the variability in leaf curvature in remains unclear. We tested the hypothesis that the leaf curvature of is affected by miRNA levels. On the basis of leaf phenotyping, 56 accessions were classified into five leaf curvature types, some of which were comparable to miRNA mutants of in phenotype. Higher levels of miR166 and miR319a expression were associated with downward curvature and wavy margins, respectively. Overexpression of the - gene caused rosette leaves to change from flat to downward curving and folding leaves to change from upward curving to flat, leading to the decrease in the number of incurved leaves and size of the leafy head. Our results reveal that miRNAs affect the types of leaf curvature in . These findings provide insight into the relationship between miRNAs and variation in leaf curvature.
许多蔬菜作物具有其典型的叶片弯曲类型。在胁迫条件下种植时，叶片向正确方向且弯曲程度合适对于绿色蔬菜产品的产量和品质很重要。最近的研究揭示了miRNA在作物中的一些作用，比如它们如何调节叶球起始的时间和叶球的形状。然而，蔬菜叶片弯曲度变异的分子机制仍不清楚。我们检验了蔬菜叶片弯曲度受miRNA水平影响这一假设。基于叶片表型分析，56份蔬菜种质被分为五种叶片弯曲类型，其中一些在表型上与miRNA突变体相当。miR166和miR319a表达水平升高分别与向下弯曲和波浪状叶缘有关。蔬菜基因的过表达导致莲座叶从平展变为向下弯曲，折叠叶从向上弯曲变为平展，从而导致内弯叶数量减少和叶球大小减小。我们的结果表明，miRNA影响蔬菜叶片弯曲的类型。这些发现为miRNA与叶片弯曲度变异之间的关系提供了见解。
