Department of Soil Science, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, 97119-900, Brazil.
Department of Forest Engineering, Federal University of Santa Maria, Frederico Westphalen, Rio Grande do Sul, 98400-000, Brazil.
Environ Sci Pollut Res Int. 2018 May;25(13):12663-12677. doi: 10.1007/s11356-018-1533-1. Epub 2018 Feb 21.
The phytoremediation of copper (Cu)-contaminated sandy soils can be influenced by the addition of vermicompost to the soil and the mycorrhization of plants. The objective of this study was to evaluate the effects of inoculation with the mycorrhizal fungus Rhizophagus clarus and the addition of different doses of bovine manure vermicompost on the phytoremediation of a sandy soil with a high Cu content using Canavalia ensiformis. Soil contaminated with 100 mg kg Cu received five doses of vermicompost and was cultivated with C. ensiformis, with and without inoculation with mycorrhizal fungus, and the Cu and nutrients in the soil and soil solution were evaluated. The concentrations of Cu and other nutrients and the biomass and Cu phytotoxicity in the plants were quantified by gauging the photochemical efficiency, concentration of photosynthetic pigments and activity of oxidative stress enzymes. The vermicompost increased the soil pH and nutrient concentrations and reduced the Cu content of the solution. When the vermicompost was applied at a dose equivalent to 80 mg phosphorus (P) kg, the phytoextraction efficiency was higher, but the phytostabilization efficiency was higher for vermicompost doses of 10 and 20 mg P kg. The presence of mycorrhizal fungi increased Cu phytostabilization, especially at vermicompost doses of 10 and 20 mg P kg. The use of vermicompost at low doses and inoculation with mycorrhizal fungi increase the phytostabilization potential of C. ensiformis in sandy soil contaminated by Cu.
添加蚯蚓粪和植物菌根共生可以影响铜（Cu）污染沙质土壤的植物修复。本研究的目的是评估接种菌根真菌亮隔孢伏革菌（Rhizophagus clarus）和添加不同剂量牛粪蚯蚓粪对高铜含量沙质土壤的修复效果，使用四棱豆（Canavalia ensiformis）作为修复植物。污染土壤中 Cu 含量为 100 mg kg，接收五剂量的蚯蚓粪，并与未接种菌根真菌的四棱豆共同种植，以及接种菌根真菌的四棱豆，同时评估土壤和土壤溶液中的 Cu 和养分含量。通过测量光化学效率、光合色素浓度和氧化应激酶活性来量化 Cu 和其他养分浓度以及植物生物量和 Cu 植物毒性。蚯蚓粪增加了土壤 pH 值和养分浓度，降低了溶液中的 Cu 含量。当蚯蚓粪施用量相当于 80 mg 磷（P）kg 时，植物提取效率更高，但当蚯蚓粪施用量为 10 和 20 mg P kg 时，植物稳定效率更高。菌根真菌的存在增加了 Cu 的植物稳定化，尤其是在蚯蚓粪施用量为 10 和 20 mg P kg 时。低剂量使用蚯蚓粪和接种菌根真菌可以增加 Cu 污染沙质土壤中四棱豆的植物稳定化潜力。
