使用RNA原位杂交技术检测临床样本中的人乳头瘤病毒E6/E7信使核糖核酸
Detection of HPV E6/E7 mRNA in Clinical Samples Using RNA In Situ Hybridization.
作者信息
Pandey Manishkumar, Bhosale Priyanka G, Mahimkar Manoj B
机构信息
Cancer Research Institute, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
Homi Bhabha National Institute, Training school complex, Anushakti Nagar, Mumbai, 400085, India.
出版信息
Methods Mol Biol. 2018;1726:167-175. doi: 10.1007/978-1-4939-7565-5_15.
Detection of human papilloma virus (HPV) in tissue specimens has been a clinical challenge since last 2 decades; however, screening for presence of E6/E7 transcripts is regarded as the gold standard, and it verifies the active HPV infection. Here, we describe "RNAscope assay" a novel RNA in situ hybridization (ISH) technology; which detects E6/E7 mRNA of seven high risk HPV subtypes (HPV 16, 18, 31, 33, 35, 52, and 58) in formalin fixed paraffin embedded (FFPE) tissue samples.
在过去二十年中，检测组织样本中的人乳头瘤病毒（HPV）一直是一项临床挑战；然而，筛查E6/E7转录本的存在被视为金标准，它可验证HPV的活跃感染。在此，我们描述一种新型的RNA原位杂交（ISH）技术——“RNAscope检测法”；该技术可在福尔马林固定石蜡包埋（FFPE）组织样本中检测七种高危HPV亚型（HPV 16、18、31、33、35、52和58）的E6/E7 mRNA。
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