Yu-Xi Ge, Lian-He Zhang, Gen Yan, Jian-Feng Zhang, Yong-Ming Pan, Ying-Hua Xuan
Affiliated Hospital of Jiangnan University, Wuxi 214000, China.
School of Public Health, Jiangnan University, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2017 Jun 26;29(5):554-558. doi: 10.16250/j.32.1374.2017043.
To establish an experimental model of acute cerebral schistosomiasis japonica and explore the MRI manifestations of acute cerebral schistosomiasis.
Rabbits were divided into 3 groups with 10 rabbits in each group. The rabbits in the experimental group were directly injected with suspension fluid of eggs (0.9 mg, 1 ml) by the cranial drilling method, those in the negative control group were given saline (1 ml) by the same method above-mentioned, and those in the blank control group were not given any treatment. Antibiotic was given to the first two groups after the operation. The clinical manifestations of the 3 groups were observed, and the magnetic resonance imaging (MRI) was performed in 30 days post-operation, and then the brain tissues were taken for pathological examinations.
All the rabbits in the experimental group exhibited inappetence, various neurological symptoms including hemiplegia, and weight loss after the operation; while those in the negative control group showed inappetence in 3 days after the operation, and 1 week later, the symptom disappeared; there were no adverse reactions in the blank control group. MRI of the experimental group showed nodular or patchy enhancement on T1WI enhancement, brain edema, abnormal ventricular dilatation, and needle augmentation. SWI displayed hypointense in the abnormal enhanced nodules and flaky hypointense on the operation brain. In the negative control group, 2 rabbits showed abnormal enhancement of the needle canal, and 1 showed mild dilatation of the ventricle. The blank control group showed normal manifestations. The pathological examinations showed abnormal appearances in 10 rabbits of the experimental group, including 6 with egg granuloma nodules, nonspecific granuloma nodules coexisted with perivascular inflammation; no granuloma nodules were found in the negative control group, but 2 rabbits showed vascular inflammation; the blank control group showed the normal brain tissue.
An experimental model of acute cerebral schistosomiasis is successfully established in rabbits by intracranial injection of schistosome eggs. The MRI examination combined with the clinical manifestations can improve the accuracy of early diagnosis of cerebral schistosomiasis.
建立急性脑型血吸虫病实验模型，探讨急性脑型血吸虫病的MRI表现。
将家兔分为3组，每组10只。实验组家兔采用颅骨钻孔法直接注射虫卵悬液（0.9mg，1ml），阴性对照组采用上述相同方法给予生理盐水（1ml），空白对照组不做任何处理。前两组术后给予抗生素。观察3组家兔的临床表现，术后30天进行磁共振成像（MRI）检查，然后取脑组织进行病理检查。
实验组家兔术后均出现食欲不振、偏瘫等多种神经症状及体重减轻；阴性对照组家兔术后3天出现食欲不振，1周后症状消失；空白对照组无不良反应。实验组MRI表现为T1WI增强呈结节状或片状强化、脑水肿、脑室异常扩张及针道强化。SWI显示异常强化结节呈低信号，术区脑片呈片状低信号。阴性对照组2只家兔针道异常强化，1只脑室轻度扩张。空白对照组表现正常。病理检查显示实验组10只家兔有异常表现，其中6只出现虫卵肉芽肿结节，非特异性肉芽肿结节与血管周围炎症并存；阴性对照组未发现肉芽肿结节，但2只家兔有血管炎症；空白对照组脑组织正常。
通过颅内注射血吸虫卵成功建立了家兔急性脑型血吸虫病实验模型。MRI检查结合临床表现可提高脑型血吸虫病早期诊断的准确性。
