Goc Zofia, Szaroma Waldemar, Kapusta Edyta, Dziubek Karol
Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow, Podbrzezie 3, 31-054 Cracow, Poland.
Chin J Physiol. 2017 Feb 28;60(1):1-10. doi: 10.4077/CJP.2017.BAF435.
Sodium nitroprusside (SNP) is an antihypertensive drug with proven dose-dependent toxic effects attributed mainly to the production of cyanide but also excesive nitric oxide (NO) and derived reactive species. The present study evaluated whether melatonin administration would have time-dependent protective effect against SNP−induced toxicity. Male Swiss mice were used in this study. Control mice were treated with 0.9% NaCl; the second group was injected with 10 mg melatonin (MEL)/kg body weight (b.w.); the third group was given SNP at the dose of 3,6 mg/kg b.w.; the fourth group received both MEL and SNP at the same doses. In homogenates of brain, liver and kidneys, activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were estimated after 3, 6 and 24 h of drugs administration. The concentration of reduced glutathione (GSH) was also evaluated in the blood, brain, liver and kidneys of mice at the same time intervals. In animals receiving MEL, the highest levels of GSH were observed in all the organs as compared to the control after 3, 6 h. Meanwhile, SNP decreased GSH concentration in the blood, brain, liver and kidneys in all time intervals. Administration of MEL in combination with SNP increased the GSH levels in all organs, as compared to the administration of SNP alone; this effect was observed after 3, 6 and 24 h. The activity of SOD, CAT and GSH-Px in the MEL-treated group increased after 3 h in all the organs, while in liver and kidney the increase was also observed after 6 h. Conversely, the SNP intoxication caused a decrease of the activity of enzymes in the tested organs in all intervals, while administration of MEL + SNP resulted in increased activities of SOD, CAT and GSH-Px in all the organs after 3 h and 6 h. The investigation carried out in the present study provide new data to add to the study of antioxidant properties of MEL and SNP-induced oxidative stress with regard to time-dependent properties in different types of tissues.
硝普钠（SNP）是一种降压药，已证实其具有剂量依赖性毒性作用，主要归因于氰化物的产生，但也与过量的一氧化氮（NO）及其衍生的活性物质有关。本研究评估了褪黑素给药是否会对SNP诱导的毒性产生时间依赖性保护作用。本研究使用雄性瑞士小鼠。对照组小鼠用0.9%氯化钠处理；第二组注射10毫克褪黑素（MEL）/千克体重（b.w.）；第三组给予3.6毫克/千克b.w.剂量的SNP；第四组接受相同剂量的MEL和SNP。在给药3、6和24小时后，估计脑、肝和肾匀浆中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。同时，在相同时间间隔评估小鼠血液、脑、肝和肾中还原型谷胱甘肽（GSH）的浓度。在接受MEL的动物中，与对照组相比，在3、6小时后所有器官中均观察到最高水平的GSH。同时，SNP在所有时间间隔内均降低了血液、脑、肝和肾中的GSH浓度。与单独给予SNP相比，MEL与SNP联合给药增加了所有器官中的GSH水平；在3、6和24小时后均观察到这种效果。MEL处理组中所有器官的SOD、CAT和GSH-Px活性在3小时后增加，而在肝和肾中6小时后也观察到增加。相反，SNP中毒在所有时间间隔内均导致受试器官中酶活性降低，而MEL + SNP给药在3小时和6小时后导致所有器官中SOD、CAT和GSH-Px活性增加。本研究进行的调查提供了新的数据，以补充关于MEL的抗氧化特性以及SNP诱导的氧化应激在不同类型组织中的时间依赖性特性的研究。
