Wen-Qiao Huang, Yuan-Jian Zhu, Da-Bing Lv, Xia Zhou, Ying-Nan Yang, Hong-Xiang Zhu-Ge
Department of Pathogenic Biology, Soochow University, Suzhou 215123, China.
Department of Clinical Laboratory, The Central Hospital of Zaozhuang Mining Group, Shandong Province, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 May 24;28(3):275-280. doi: 10.16250/j.32.1374.2015171.
To explore the correlation between the genetic dissimilarity and heterozygosity of mates and the pathogenicity of in the definitive host.
By using seven microsatellite loci markers, genotyping of sixteen pairs randomly mated was performed, the genetic dissimilarity and heterozygosity were calculated between the mates, and the correlation between the genetic dissimilarity and heterozygosity of the mates and the pathogenicity of in the definitive host was evaluated.
There was a significant correlation between the genetic similarity of mates and the mean number of eggs per worm pair in the liver and intestinal tissue ( = 0.501 6, < 0.05; = 0.796 5, < 0.01, respectively) and the hatching rate of deposited eggs in the liver ( = 0.508 3, < 0.05), respectively. There was no correlation between the genetic similarity of the mates and hepatosplenomegaly per worm pair ( = 0.109 5, > 0.05; = 0.265 3, > 0.05, respectively) and the average diameter of granuloma in the liver ( = -0.272 7, > 0.05), respectively. There was no correlation between the heterozygosity of the mates and all the pathological parameters of in the definitive host ( > 0.05).
There is the correlation between the genetic dissimilarity of the mates and the pathogenicity of in the definitive host, and the genetic dissimilarity is greater, pathogenicity is weaker. There is no correlation between heterozygosity of the mates and the pathogenicity of in the definitive host.
探讨配偶的基因差异和杂合性与终末宿主中[具体病原体名称未给出]致病性之间的相关性。
利用7个微卫星位点标记，对16对随机交配的[具体生物名称未给出]进行基因分型，计算配偶之间的基因差异和杂合性，并评估配偶的基因差异和杂合性与终末宿主中[具体病原体名称未给出]致病性之间的相关性。
[具体生物名称未给出]配偶的基因相似性与肝脏和肠道组织中每对虫体的平均产卵数（分别为r = 0.501 6，P < 0.05；r = 0.796 5，P < 0.01）以及肝脏中产出卵的孵化率（r = 0.508 3，P < 0.05）之间分别存在显著相关性。配偶的基因相似性与每对虫体的肝脾肿大（分别为r = 0.109 5，P > 0.05；r = 0.265 3，P > 0.05）以及肝脏中肉芽肿的平均直径（r = -0.272 7，P > 0.05）之间分别不存在相关性。配偶的杂合性与终末宿主中[具体病原体名称未给出]的所有病理参数之间均不存在相关性（P > 0.05）。
配偶的基因差异与终末宿主中[具体病原体名称未给出]的致病性之间存在相关性，基因差异越大，致病性越弱。配偶的杂合性与终末宿主中[具体病原体名称未给出]的致病性之间不存在相关性。
