Zi-Gang Zhang, Xiao-Mei Chen, Dan-Hua Su, Yuan Liu, Tao Fu, Jia-Miao Duan-Mu, Liang Wu, Xu-Gan Jiang, Sheng-Xia Chen, Jian-Ping Cao
School of Medicine, Jiangsu University, Zhenjiang 212013, China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 Apr 25;28(3):289-292. doi: 10.16250/j.32.1374.2016077.
To clone and express the thioredoxin (Trx) from RH strain tachyzoites of , establish the prokaryotic expression vector and purify the recombinant protein, then produce the polyclonal anti-Trx antibody in rabbits.
Trx fragment was amplified by PCR and cloned into the pET-28a (+) vector, and the recombinant protein was induced with IPTG and purified by Ni-NTA affinity chromatography. The polyclonal antibody specificity was detected by Western blotting.
The trx gene was amplified from cDNA by PCR. The recombinant plasmid /pET-28a (+) was usefully constructed, and the recombinant TRX protein was expressed and purified. The TRX polyclonal antibody was also obtained. The specific band of TRX was detected by Western blotting.
Western blotting can detect the specificity of polyclonal anti-Trx antibody, which will facilitate the biological functions of Trx.
