Sailakshmi G, Mitra Tapas, Gnanamani A
Microbiology Division, Central Leather Research Institute (CSIR, New Delhi), Adyar, Chennai, 20, Tamil Nadu, India.
Prog Biomater. 2013 Apr 23;2(1):11. doi: 10.1186/2194-0517-2-11.
Transformation of natural polymers to three-dimensional (3D) scaffolds for biomedical applications faces a number of challenges, viz., solubility, stability (mechanical and thermal), strength, biocompatibility, and biodegradability. Hence, intensive research on suitable agents to provide the requisite properties has been initiated at the global level. In the present study, an attempt was made to engineer chitosan and collagen macromolecules using sebacic acid, and further evaluation of the mechanical stability and biocompatible property of the engineered scaffold material was done. A 3D scaffold material was prepared using chitosan at 1.0% (w/v) and sebacic acid at 0.2% (w/v); similarly, collagen at 0.5% (w/v) and sebacic acid at 0.2% (w/v) were prepared individually by freeze-drying technique. Analysis revealed that the engineered scaffolds displayed an appreciable mechanical strength and, in addition, were found to be biocompatible to NIH 3T3 fibroblast cells. Studies on the chemistry behind the interaction and the characteristics of the cross-linked scaffold materials suggested that non-covalent interactions play a major role in deciding the property of the said polymer materials. The prepared scaffold was suitable for tissue engineering application as a wound dressing material.
将天然聚合物转化为用于生物医学应用的三维（3D）支架面临许多挑战，即溶解性、稳定性（机械和热稳定性）、强度、生物相容性和生物降解性。因此，全球范围内已开始对提供所需性能的合适试剂进行深入研究。在本研究中，尝试使用癸二酸对壳聚糖和胶原蛋白大分子进行改造，并对改造后的支架材料的机械稳定性和生物相容性进行了进一步评估。使用1.0%（w/v）的壳聚糖和0.2%（w/v）的癸二酸制备了一种3D支架材料；同样，通过冷冻干燥技术分别制备了0.5%（w/v）的胶原蛋白和0.2%（w/v）的癸二酸。分析表明，改造后的支架显示出可观的机械强度，此外，还发现对NIH 3T3成纤维细胞具有生物相容性。对相互作用背后的化学原理和交联支架材料特性的研究表明，非共价相互作用在决定上述聚合物材料的性能方面起主要作用。所制备的支架作为伤口敷料材料适用于组织工程应用。
