Microbiology Division, Central Leather Research Institute (CSIR, New Delhi), Adyar, Chennai 600020, Tamil Nadu, India.
J Mater Sci Mater Med. 2012 May;23(5):1309-21. doi: 10.1007/s10856-012-4586-6. Epub 2012 Feb 26.
The present study emphasizes the influence of non-covalent interactions on the mechanical and thermal properties of the scaffolds of chitosan/collagen origin. Malonic acid (MA), a bifuncitonal diacid was chosen to offer non-covalent cross-linking. Three dimensional scaffolds was prepared using chitosan at 1.0% (w/v) and MA at 0.2% (w/v), similarly collagen 0.5% (w/v) and MA 0.2% (w/v) and characterized. Results on FT-IR, TGA, DSC, SEM and mechanical properties (tensile strength, stiffness, Young's modulus, etc.) assessment demonstrated the existence of non-covalent interaction between MA and chitosan/collagen, which offered flexibility and high strength to the scaffolds suitable for tissue engineering research. Studies using NIH 3T3 fibroblast cells suggested biocompatibility nature of the scaffolds. Docking simulation study further supports the intermolecular hydrogen bonding interactions between MA and chitosan/collagen.
本研究强调了非共价相互作用对壳聚糖/胶原来源支架的机械和热性能的影响。选择了马来酸（MA）作为双功能二羧酸，以提供非共价交联。使用 1.0%（w/v）壳聚糖和 0.2%（w/v）MA 以及 0.5%（w/v）胶原蛋白和 0.2%（w/v）MA 制备了三维支架，并对其进行了表征。FT-IR、TGA、DSC、SEM 和机械性能（拉伸强度、刚度、杨氏模量等）评估的结果表明，MA 与壳聚糖/胶原蛋白之间存在非共价相互作用，这为支架提供了柔韧性和高强度，适用于组织工程研究。使用 NIH 3T3 成纤维细胞的研究表明了支架的生物相容性。对接模拟研究进一步支持了 MA 与壳聚糖/胶原蛋白之间的分子间氢键相互作用。
