a Department of Ophthalmology and Visual Sciences , University of Louisville , Louisville , Kentucky , USA.
b Division of Ophthalmology , Cook County Health and Hospitals System , Chicago , Illinois , USA.
Ocul Immunol Inflamm. 2019;27(4):578-582. doi: 10.1080/09273948.2018.1431291. Epub 2018 Feb 22.
: To compare genetic testing for microbes in infectious endophthalmitis or uveitis to culture.: This was a retrospective, single-center case series that enrolled patients with clinically suspected endophthalmitis or uveitis of unknown etiology. Aqueous humor or vitreous was collected and sent for routine cultures and genetic testing.: In total, 46 patients were enrolled. Genetic testing was positive in 32/46 (70%) cases and culture 6/46 cases (13%). Five of 16 uveitis cases had a final clinical diagnosis of infectious uveitis, and polymerase chain reaction (PCR) was positive in 4/5 cases (80%), versus 0% for culture. In uveitis cases, PCR was 80% sensitive and 82% specific, and culture had 0% sensitivity. The overall sensitivity and specificity of PCR for all cases were 85% and 67%, respectively, compared with 17% and 100% for culture.: Genetic assays are inexpensive ($25/case) and more sensitive than culture for identifying intraocular pathogens in endophthalmitis and uveitis.
这是一项回顾性、单中心病例系列研究，纳入了临床疑似病因不明的眼内炎或葡萄膜炎患者。收集房水或玻璃体液并进行常规培养和基因检测。共纳入 46 例患者。基因检测阳性 32/46（70%）例，培养阳性 6/46 例（13%）。16 例葡萄膜炎中有 5 例最终临床诊断为感染性葡萄膜炎，聚合酶链反应（PCR）阳性 4/5 例（80%），而培养为 0%。在葡萄膜炎病例中，PCR 的敏感性为 80%，特异性为 82%，而培养的敏感性为 0%。与培养的敏感性为 17%和特异性为 100%相比，PCR 对所有病例的总体敏感性和特异性分别为 85%和 67%。
基因检测比培养更便宜（每个病例 25 美元），且在鉴定眼内炎和葡萄膜炎的眼内病原体方面比培养更敏感。
