Zhang Qi, Li Kewen, Yan Jinhong, Wang Zhuo, Wu Qi, Bi Long, Yang Min, Han Yisheng
Department of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, PR China; Department of Orthopedics, No. 323 Hospital of Chinese PLA, Xi'an, PR China.
Department of Orthopedics, Qinghai University Affiliated Hospital, Xining, PR China.
Biochem Biophys Res Commun. 2018 Mar 18;497(4):1011-1017. doi: 10.1016/j.bbrc.2018.02.152. Epub 2018 Feb 19.
The objective was to investigate whether a graphene coating could improve the surface bioactivity of a cobalt-chromium-molybdenum-based alloy (CoCrMo). Graphene was produced by chemical vapor deposition and transferred to the surface of the CoCrMo alloy using an improved wet transfer approach. The morphology of the samples was observed, and the adhesion force and stabilization of graphene coating were analyzed by a nanoscratch test and ultrasonication test. In an in vitro study, the adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) cultured on the samples were quantified via an Alamar Blue assay and cell counting kit-8 (CCK-8) assay. The results showed that it is feasible to apply graphene to modify the surface of a CoCrMo alloy, and the enhancement of the adhesion and proliferation of BMSCs was also shown in the present study. In conclusion, graphene exhibits considerable potential for enhancing the surface bioactivity of CoCrMo alloy.
目的是研究石墨烯涂层是否能改善钴铬钼基合金（CoCrMo）的表面生物活性。通过化学气相沉积法制备石墨烯，并采用改进的湿法转移方法将其转移到CoCrMo合金表面。观察样品的形貌，并通过纳米划痕试验和超声处理试验分析石墨烯涂层的附着力和稳定性。在一项体外研究中，通过Alamar Blue检测法和细胞计数试剂盒-8（CCK-8）检测法定量分析在样品上培养的骨髓间充质干细胞（BMSC）的黏附与增殖情况。结果表明，将石墨烯应用于CoCrMo合金表面改性是可行的，并且本研究还表明BMSC的黏附与增殖得到了增强。总之，石墨烯在增强CoCrMo合金表面生物活性方面具有巨大潜力。
