Adibi-Motlagh Behzad, Lotfi Abbas Sahebghadam, Rezaei Aram, Hashemi Ehsan
Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2018 Jan 1;82:323-329. doi: 10.1016/j.msec.2017.05.039. Epub 2017 May 17.
The immobilization of bioactive peptides as key molecules in numerous biological and physiological functions holds promise for designing advanced biomaterials. Graphene and its derivatives, having unique physicochemical properties, have brought considerable attention in the life sciences. In this regard, the chemical manipulation of the graphene surface with bioactive peptides opens a new horizon to design bioactive materials for a variety of future nanobiotechnologies. In this study, the first straightforward strategy for the covalent immobilization of the cell-adhesion peptide onto the graphene surface based on the Ugi four-component assembly process (Ugi 4-CAP) will be presented. The modified adhesion motif peptide, as an amine component in the presence of formaldehyde, cyclohexylisocyanide and carboxylated-graphene (G-COOH), was adopted in a four component reaction to fabricate a peptide-graphene (Peptide-G) biomaterial in water as a green solvent at an ambient temperature. The amino functional groups corresponded to the modified adhesion motif peptide and were immobilized onto the graphene sheets, which were quantified by the Kaiser test. The sheets were characterized by further analyses with FT-IR, AFM, UV-vis, Raman and thermogravimetric analyses. The Peptide-G biomaterial showed excellent biocompatibility. In addition, the Peptide-G treated surface, due to the presence of RGD on the surface of the graphene, significantly accelerated the proliferation of human mesenchymal stem cells (hMSCs) at a better rate regarding the tissue plate.
生物活性肽作为众多生物和生理功能中的关键分子，其固定化有望用于设计先进的生物材料。石墨烯及其衍生物具有独特的物理化学性质，在生命科学领域引起了广泛关注。在这方面，用生物活性肽对石墨烯表面进行化学修饰，为设计适用于各种未来纳米生物技术的生物活性材料开辟了新的前景。在本研究中，将介绍基于Ugi四组分组装过程（Ugi 4-CAP）将细胞粘附肽共价固定在石墨烯表面的首个直接策略。在甲醛、环己基异氰化物和羧基化石墨烯（G-COOH）存在的情况下，将修饰后的粘附基序肽作为胺组分，在四组分反应中采用，以水作为绿色溶剂，在环境温度下制备肽-石墨烯（Peptide-G）生物材料。与修饰后的粘附基序肽对应的氨基官能团被固定在石墨烯片上，通过凯氏试验对其进行定量。通过傅里叶变换红外光谱（FT-IR）、原子力显微镜（AFM）、紫外可见光谱（UV-vis）、拉曼光谱和热重分析等进一步分析对这些片材进行表征。Peptide-G生物材料表现出优异的生物相容性。此外，由于石墨烯表面存在RGD，经Peptide-G处理的表面显著加速了人间充质干细胞（hMSCs）的增殖，与组织培养板相比增殖速率更高。
