Al-Baloushi Amna E, Pál Tibor, Ghazawi Akela, Sonnevend Agnes
1 Department of Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University , Al Ain, United Arab Emirates.
2 Microbiology Laboratory, Al Ain Hospital , Al Ain, United Arab Emirates.
Acta Microbiol Immunol Hung. 2018 Jun 1;65(2):135-150. doi: 10.1556/030.65.2018.005. Epub 2018 Feb 23.
Enterobacteriaceae co-producing NDM- and OXA-48-type carbapenemases were encountered in higher frequency in the United Arab Emirates (UAE) than in the neighboring countries in our earlier study. The aim of this investigation was to characterize the seven double carbapenemase producer Klebsiella pneumoniae found in the region to assess factors contributing to their emergence. Three K. pneumoniae ST14 isolated in the UAE harboring bla on IncHI1b and bla on IncColE plasmids were clonally related. Furthermore, two K. pneumoniae from the UAE, ABC106 and ABC137 belonged to ST307 and ST1318, respectively. ABC106 carried bla on IncHI1b, and bla on IncL/M plasmids, whereas ABC137 possessed bla on IncX3 and bla on IncL/M plasmids. The double carbapenemase-producing K. pneumoniae from Oman (OMABC109) and Saudi Arabia (SA54) belonged to ST11 and ST152, respectively. OMABC109 harbored bla on an IncHI1b plasmid highly similar to the NDM-plasmid of ABC106 and carried a chromosomally coded bla located on Tn2013. SA54 possessed a bla on an IncFIb/FII plasmid and a bla on an IncL/M plasmid. Based on these findings, clonal spread and horizontal transfer of carbapenemase genes located on transposons or self-transmissible plasmids contributed equally to the emergence of double carbapenemase-producing Enterobacteriaceae in the region.
在我们早期的研究中，发现产NDM和OXA - 48型碳青霉烯酶的肠杆菌科细菌在阿拉伯联合酋长国（UAE）出现的频率高于邻国。本研究的目的是对该地区发现的7株产双碳青霉烯酶的肺炎克雷伯菌进行特征分析，以评估导致其出现的因素。在阿联酋分离出的3株肺炎克雷伯菌ST14，携带位于IncHI1b质粒上的bla和位于IncColE质粒上的bla，它们具有克隆相关性。此外，来自阿联酋的2株肺炎克雷伯菌ABC106和ABC137分别属于ST307和ST1318。ABC106在IncHI1b质粒上携带bla，在IncL/M质粒上携带bla，而ABC137在IncX3质粒上拥有bla，在IncL/M质粒上拥有bla。来自阿曼（OMABC109）和沙特阿拉伯（SA54）的产双碳青霉烯酶的肺炎克雷伯菌分别属于ST11和ST152。OMABC109在一个与ABC106的NDM质粒高度相似的IncHI1b质粒上携带bla，并携带位于Tn2013上的染色体编码bla。SA54在一个IncFIb/FII质粒上拥有bla，在一个IncL/M质粒上拥有bla。基于这些发现，位于转座子或自我传递质粒上的碳青霉烯酶基因的克隆传播和水平转移对该地区产双碳青霉烯酶肠杆菌科细菌的出现起到了同等作用。
