Knáb Mónika, Szili-Kovács Tibor, Márialigeti Károly, Móga János, Borsodi Andrea K
1 Department of Microbiology, ELTE Eötvös Loránd University , Budapest, Hungary.
2 Centre for Agricultural Research, Institute for Soil Sciences and Agricultural Chemistry, Hungarian Academy of Sciences , Budapest, Hungary.
Acta Microbiol Immunol Hung. 2018 Dec 1;65(4):439-458. doi: 10.1556/030.65.2018.002. Epub 2018 Feb 23.
Karst areas have great environmental importance as sources of subsurface water and often maintain very sensitive ecosystems. In recent years, increasing number of microbiological studies focused on the bacterial communities of karst soils. In this study, diversity examinations on two distinct Hungarian karst areas, Aggtelek and Tapolca, were performed using parallel cultivation and molecular cloning methods. The phylogenetic affiliation of bacterial strains and molecular clones was determined based on their 16S rRNA gene sequences. Bacterial isolates were identified as members of the phyla Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Besides the taxa identified by cultivation, members of the phyla Chloroflexi, Cyanobacteria, Acidobacteria, Verrucomicrobia, and Gemmatimonadetes were detected by the cloning. The difference in the composition of soil bacterial communities was related to geographic locations and soil types. Both the highest and the lowest bacterial diversities were detected in samples from Aggtelek National Park, characterized by Leptic Luvisol and Rendzic Leptosol soil types. The difference in the composition of bacterial communities between Rendzic Leptosol and Leptic Phaeozem soil types at Tapolca could be the result of human impacts.
岩溶地区作为地下水的来源具有重要的环境意义，并且常常维持着非常敏感的生态系统。近年来，越来越多的微生物学研究聚焦于岩溶土壤中的细菌群落。在本研究中，使用平行培养和分子克隆方法对匈牙利两个不同的岩溶地区——阿格泰莱克和陶波尔曹进行了多样性检测。基于细菌菌株和分子克隆的16S rRNA基因序列确定了它们的系统发育归属。细菌分离株被鉴定为放线菌门、厚壁菌门、变形菌门和拟杆菌门的成员。除了通过培养鉴定出的分类群外，通过克隆还检测到了绿弯菌门、蓝细菌门、酸杆菌门、疣微菌门和芽单胞菌门的成员。土壤细菌群落组成的差异与地理位置和土壤类型有关。在以薄层淋溶土和黑色石灰土土壤类型为特征的阿格泰莱克国家公园的样本中检测到了最高和最低的细菌多样性。陶波尔曹地区黑色石灰土和薄层黑土土壤类型之间细菌群落组成的差异可能是人类影响的结果。
