Orosz Erika, Szentmáry Nóra, Kiss Huba J, Farkas Ágnes, Kucsera István, Nagy Zoltán Zsolt
1 Department of Parasitology, National Public Health Institute , Budapest, Hungary.
2 Department of Ophthalmology, Semmelweis University , Budapest, Hungary.
Acta Microbiol Immunol Hung. 2018 Mar 1;65(1):73-79. doi: 10.1556/030.65.2018.007. Epub 2018 Feb 23.
Acanthamoeba has a worldwide distribution in the environment and it is capable of causing a painful sight-threatening disease of the cornea designated as Acanthamoeba keratitis (AK). Nowadays, the cases of AK have surged all over the world along with its disease burden due to increasing use of contact lenses used not only for optical correction but also for cosmetic purposes. In our present work, epithelial abrasion of a 27-year-old female soft contact lens wearer with keratitis was examined. Genotype identification was carried out with a real-time fluorescence resonance energy transfer polymerase chain reaction (PCR) assay based on sequence analysis of the 18S rRNA gene. Genotyping allowed the identification of a T8 group isolate. The analysis confirmed the importance of a complete diagnostic protocol, including a PCR assay, for the clinical diagnosis of AK from human samples. Acanthamoeba T8 should be considered as potential causative organism in keratitis in human.
棘阿米巴在全球环境中均有分布，它能够引发一种疼痛的、威胁视力的角膜疾病，称为棘阿米巴角膜炎（AK）。如今，随着用于光学矫正以及美容目的的隐形眼镜使用量增加，全球范围内AK病例及其疾病负担都出现了激增。在我们目前的工作中，对一名患有角膜炎的27岁女性软性隐形眼镜佩戴者的角膜上皮擦伤情况进行了检查。基于18S rRNA基因序列分析，采用实时荧光共振能量转移聚合酶链反应（PCR）检测法进行基因型鉴定。基因分型鉴定出一株T8组分离株。分析证实了包括PCR检测法在内的完整诊断方案对于从人体样本中临床诊断AK的重要性。棘阿米巴T8应被视为人类角膜炎的潜在致病生物。
