Martyniak Marcin, Franczak Anita, Kotwica Genowefa
University of Warmia and Mazury in Olsztyn, Department of Animal Physiology Oczapowskiego 1A, 10-719, Olsztyn, Poland.
University of Warmia and Mazury in Olsztyn, Department of Animal Physiology Oczapowskiego 1A, 10-719, Olsztyn, Poland.
Reprod Biol. 2018 Jun;18(2):143-150. doi: 10.1016/j.repbio.2018.02.002. Epub 2018 Feb 19.
Past studies of the oviducts have documented oviductal steroid production during the oestrous cycle in pigs. The present study examined whether the pig oviducts are the source of steroid hormones during early pregnancy. In the ampulla and isthmus, the expression of 3β-hydroxysteroid dehydrogenase (3βHSD) and aromatase cytochrome P450 (CYP19) mRNA by real-time PCR, cellular localization and quantities of the studied proteins by immunofluorescence and Western blot analysis, and concentration of steroid hormones in oviductal flushings by radioimmunoassay, were studied. The expression of 3βHSD in the ampulla and isthmus was correlated (r = 0.89) and higher on Days 2-3 and 15-16 than on Days 10-11 and 12-13. CYP19 expression was elevated in the ampulla on Days 2-3, 10-11 and 15-16 and in the isthmus on Days 2-3 vs. the other days studied. The studied proteins were localized in oviductal epithelial cells. In the ampulla, the quantity of 3βHSD protein did not change, and was greater in the isthmus on Days 2-3 vs. Days 12-13 of pregnancy. The P450arom protein quantity increased in the ampulla on Days 2-3 vs. Days 10-11 and 15-16 and vs. Days 10-11 and 12-13 in the isthmus. The concentrations of progesterone and androstenedione in oviductal flushings were lowest on Days 12-13 and on Days 2-3 and 15-16, respectively, while oestradiol-17β and oestrone levels did not change. Porcine oviducts are the sources of steroid hormones during early pregnancy. The expression of steroidogenic enzymes primarily increases during the embryos presence in the oviduct, i.e., on Days 2-3 of pregnancy.
过去对输卵管的研究记录了猪发情周期中输卵管甾体激素的产生情况。本研究检测了猪输卵管在妊娠早期是否为甾体激素的来源。通过实时荧光定量PCR检测壶腹部和峡部中3β-羟基类固醇脱氢酶（3βHSD）和芳香化酶细胞色素P450（CYP19）mRNA的表达，通过免疫荧光和蛋白质印迹分析研究所研究蛋白质的细胞定位和数量，并通过放射免疫分析法检测输卵管冲洗液中甾体激素的浓度。壶腹部和峡部中3βHSD的表达具有相关性（r = 0.89），在第2 - 3天和第15 - 16天高于第10 - 11天和第12 - 13天。CYP19的表达在壶腹部的第2 - 3天、第10 - 11天和第15 - 16天以及峡部的第2 - 3天相对于其他研究天数有所升高。所研究的蛋白质定位于输卵管上皮细胞中。在壶腹部，3βHSD蛋白的数量没有变化，在妊娠第2 - 3天的峡部中比第12 - 13天更多。P450arom蛋白的数量在壶腹部第2 - 3天相对于第10 - 11天和第15 - 16天以及峡部第2 - 3天相对于第10 - 11天和第12 - （此处原文有误，应为13）天有所增加。输卵管冲洗液中孕酮和雄烯二酮的浓度分别在第12 - 13天以及第2 - 3天和第15 - 16天最低，而雌二醇-17β和雌酮水平没有变化。猪输卵管是妊娠早期甾体激素的来源。甾体生成酶的表达主要在胚胎存在于输卵管期间，即妊娠第2 - 3天增加。
