Smolinska N, Dobrzyn K, Kiezun M, Szeszko K, Maleszka A, Kaminski T
Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
J Physiol Pharmacol. 2016 Jun;67(3):443-56.
Adiponectin and its receptors are expressed in the human and porcine uterus and this endocrine system has important role in the regulation of reproductive processes. The expression of steroidogenic acute regulatory protein (StAR) and 3β-hydroxysteroid dehydrogenase (HSD3B1) were observed in the human and porcine uterus during the oestrous cycle and pregnancy. The de novo synthesis of steroids in the uterus might be a crucial factor for effective implantation and maintenance of pregnancy. We hypothesized that adiponectin modulates the expression of key enzymes in the synthesis of the steroids: StAR, P450 side chain cleavage enzyme (CYP11A1) and HSD3B1, as well as progesterone (P4) and androstenedione (A4) secretion by the porcine uterus. Endometrial and myometrial explants harvested from gilts (n = 5) on days 10 to 11, 12 to 13, 15 to 16 and 27 to 28 of pregnancy and on days 10 to 11 of the oestrous cycle were cultured in vitro in the presence of adiponectin (1, 10 μg/ml), adiponectin with insulin (10 ng/ml) and insulin alone (10 ng/ml). Gene expression was examined by real-time PCR, and the secretion of the steroids was determined by radioimmunoassay. The content of StAR, CYP11A1 and HSD3B1 mRNAs and the secretion of P4 and A4 was modulated by adiponectin in endometrial and myometrial tissue explants during early pregnancy and the oestrous cycle. In this action adiponectin interacted with insulin. Insulin itself also regulated the steroidogenic activity of the porcine uterus. ere we reported, for the first time, the expression of CYP11A1 genes in the porcine endometrium and myometrium. Our novel findings indicate that adiponectin affects basal and insulin-stimulated expression of key steroidogenic genes and production of steroid hormones by the porcine uterus during maternal recognition of pregnancy and implantation.
脂联素及其受体在人和猪的子宫中表达，并且这个内分泌系统在生殖过程的调节中发挥重要作用。在发情周期和妊娠期的人和猪子宫中观察到了类固醇生成急性调节蛋白（StAR）和3β-羟基类固醇脱氢酶（HSD3B1）的表达。子宫中类固醇的从头合成可能是有效着床和维持妊娠的关键因素。我们假设脂联素可调节类固醇合成中关键酶的表达：StAR、P450侧链裂解酶（CYP11A1）和HSD3B1，以及猪子宫中孕酮（P4）和雄烯二酮（A4）的分泌。从妊娠第10至11天、12至13天、15至16天和27至28天以及发情周期第10至11天的后备母猪（n = 5）采集子宫内膜和子宫肌层外植体，在脂联素（1、10μg/ml）、脂联素与胰岛素（10 ng/ml）以及单独胰岛素（10 ng/ml）存在的情况下进行体外培养。通过实时PCR检测基因表达，并通过放射免疫测定法测定类固醇的分泌。在妊娠早期和发情周期中，脂联素可调节子宫内膜和子宫肌层组织外植体中StAR、CYP11A1和HSD3B1 mRNA的含量以及P4和A4的分泌。在这一作用过程中，脂联素与胰岛素相互作用。胰岛素本身也调节猪子宫的类固醇生成活性。我们首次报道了CYP11A1基因在猪子宫内膜和子宫肌层中的表达。我们的新发现表明，在母体识别妊娠和着床期间，脂联素会影响猪子宫中关键类固醇生成基因的基础表达和胰岛素刺激的表达以及类固醇激素的产生。
