Shivakrishna P, Ashok Reddy K, Manohar Rao D
Department of Genetics, Osmania University, Hyderabad, Telangana State, India.
Synteny Lifesciences Pvt. Ltd, Hyderabad, Telangana State, India.
Saudi J Biol Sci. 2018 Feb;25(2):285-289. doi: 10.1016/j.sjbs.2017.04.008. Epub 2017 Apr 25.
Drought, one of the environmental stresses, plays crucial role in reduction in plant production on majority of agricultural fields of world, In order to evaluate drought stress on RNA content Relative water content (RWC), and chlorophyll content, Water deficit was induced by Polyethylene glycol (PEG) in peanut (), accession number ICGV 91114. In this current study we evaluate RNA content and Relative water content (RWC) both in leaves and roots and chlorophyll content in leaf. The present study was undertaken with the aim to investigate the effect of water deficit imposed by PEG-6000, 40 old day seedlings were treated with varying concentrations of polyethylene glycol-6000 (PEG-6000; w/v-5%, 10%, 15% & 20%) for 24 h. The results showed that RNA content and Relative water content (RWC) content was significantly reduced in both leaves and roots with increased concentration of PEG, In leaves, a concentration dependent decline in chlorophyll content with increasing concentration of polyethylene glycol-6000 (PEG-6000). Reduction in chlorophyll '' level was to a greater extent than the chlorophyll ''. Thus, this attributes can be used as screening tool for drought tolerance in peanut.
干旱作为环境胁迫之一，在世界上大多数农田的作物减产中起着关键作用。为了评估干旱胁迫对花生（品种登录号ICGV 91114）RNA含量、相对含水量（RWC）和叶绿素含量的影响，采用聚乙二醇（PEG）诱导水分亏缺。在本研究中，我们评估了花生叶片和根系中的RNA含量以及相对含水量（RWC），还有叶片中的叶绿素含量。本研究旨在探究PEG - 6000造成的水分亏缺的影响，用不同浓度（5%、10%、15%和20%，w/v）的聚乙二醇 - 6000（PEG - 6000）处理40日龄的幼苗24小时。结果表明，随着PEG浓度的增加，叶片和根系中的RNA含量和相对含水量（RWC）均显著降低；在叶片中，随着聚乙二醇 - 6000（PEG - 6000）浓度的增加，叶绿素含量呈浓度依赖性下降。叶绿素b含量的下降幅度大于叶绿素a。因此，这些指标可作为花生耐旱性的筛选工具。
