Pratap Veer, Sharma Yogesh Kumar
Department of Botany, Lucknow University, Lucknow - 226 007, India.
J Environ Biol. 2010 Sep;31(5):721-6.
An experiment was conducted to see the impact of osmotic stress as it is one of the main cause in various soil and water disorders in agricultural field crops, speciallythe seed germination and seedling growth. The osmotic stress was generated using PEG-6000 and the seed germination, seedling growth were evaluated including the status of pigments i.e. chlorophyll (a, b and total), total carotenoids, pheophytin (a, b and total) and different enzymes like amylase, peroxidase, catalase and superoxide dismutase. The various osmotic potentials generated (-2, -5 and -10 bars) showed significant decrease in germination percentage as at the osmotic potential of -10 bars it was observed 70 in comparison to 90% of control. All the seedling growth parameter also showed inhibition with increase in osmotic potential. Increase in osmotic stress decreased Chlorophyll 'a', while Chlorophyll 'b' was increased in -5 bars while total chlorophyll showed decrease in -5 bars osmotic potential. Total carotenoids and pheophytin (a, b and total) were highly increased in -5 bars and decreased in -10 bars osmotic concentration. Enzymatic activity was found to be decreased in amylase while peroixidase, catalase and SOD were increased at different osmotic gradients in comparison to control. The data observed in the experiment can be helpful to assess the impact of any kind of osmotic stress on plant growth and development in crops.
开展了一项实验以观察渗透胁迫的影响，因为它是农田作物各种土壤和水分问题的主要原因之一，特别是对种子萌发和幼苗生长的影响。使用聚乙二醇6000（PEG - 6000）产生渗透胁迫，并对种子萌发、幼苗生长进行评估，包括色素（即叶绿素（a、b和总量）、总类胡萝卜素、脱镁叶绿素（a、b和总量））的状况以及淀粉酶、过氧化物酶、过氧化氢酶和超氧化物歧化酶等不同酶的情况。所产生的各种渗透势（-2、-5和-10巴）显示发芽率显著下降，在-10巴的渗透势下，观察到发芽率为70%，而对照为90%。所有幼苗生长参数也随着渗透势的增加而受到抑制。渗透胁迫增加使叶绿素“a”减少，而叶绿素“b”在-5巴时增加，总叶绿素在-5巴的渗透势下减少。总类胡萝卜素和脱镁叶绿素（a、b和总量）在-5巴时大幅增加，在-10巴的渗透浓度下减少。与对照相比，淀粉酶的酶活性降低，而过氧化物酶、过氧化氢酶和超氧化物歧化酶在不同渗透梯度下增加。实验中观察到的数据有助于评估任何类型的渗透胁迫对作物植物生长和发育的影响。
