CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, s/n, 4450-238, Matosinhos, Portugal.
United States Department of Agriculture, Ames, IA, USA.
Protein J. 2019 Dec;38(6):628-639. doi: 10.1007/s10930-019-09857-0.
Queen conch (Lobatus gigas) is a marine gastropod endemic to the Caribbean. This species is a cultural symbol, being a significant local food source and the second largest commercial fishery in the region. However, over-exploitation and natural habitat degradation have exerted high survival pressure on this species. This work aims to provide novel proteomic data to highlight the metabolism of the species and to provide an important tool for the understanding of queen conch biology and physiology. Herein, we profiled the whole proteome from 3 organs (gills, digestive gland and muscle) of L. gigas combining gel-free and gel-based techniques. Overall 420 clusters of proteins were identified corresponding to the minimum identification requirement of protein sequence redundancy. Gene ontology and KEGG analysis highlighted 59 metabolic pathways between identified proteins. The most relevant routes according to the number of sequences found per pathway were purine and thiamine metabolism, closely related to nucleotide and carbohydrate metabolism. We also emphasize the high number of proteins associated to the biosynthesis of antibiotics (93 proteins and a total of 28 enzymes), which were among the top-twenty pathways identified by KEGG analysis. The proteomics approach allowed the identification and description of putative markers of oxidative stress, xenobiotic metabolism, heat shock response and respiratory chain for the first time in the species, which could be extremely useful in future investigations for diagnosing and monitoring L. gigas population health.
皇后芋螺（Lobatus gigas）是加勒比海特有的一种海洋腹足纲动物。这种物种是一种文化象征，是当地重要的食物来源，也是该地区第二大商业渔业。然而，过度开发和自然栖息地的退化给该物种带来了巨大的生存压力。这项工作旨在提供新的蛋白质组学数据，以突出该物种的新陈代谢，并为了解皇后芋螺的生物学和生理学提供重要工具。在此，我们结合无胶和胶基技术，对 3 种器官（鳃、消化腺和肌肉）的全蛋白质组进行了分析。总共鉴定了 420 个蛋白质簇，达到了蛋白质序列冗余的最低鉴定要求。基因本体论和 KEGG 分析强调了鉴定蛋白质之间的 59 个代谢途径。根据每条途径中发现的序列数量，最相关的途径是嘌呤和硫胺素代谢，与核苷酸和碳水化合物代谢密切相关。我们还强调了与抗生素生物合成相关的大量蛋白质（93 种蛋白质和总共 28 种酶），它们是 KEGG 分析确定的前 20 种途径之一。蛋白质组学方法首次在该物种中鉴定和描述了氧化应激、外来化合物代谢、热休克反应和呼吸链的潜在标志物，这对未来诊断和监测 L. gigas 种群健康的研究非常有用。
