Imamura M, Miyazaki T, Fujimoto H, Fukuhara T, Kasai M, Itaya T, Sakurada K
Transplantation. 1986 Nov;42(5):548-55. doi: 10.1097/00007890-198611000-00022.
An intrasplenic injection (i.s.) of BALB/c bone marrow cells induces a higher survival rate than an intravenous injection (i.v.) in irradiated C3H/He recipients. Coculture experiments revealed the presence of alloantigen-specific and nonspecific suppressor cells in the spleens of mice injected i.s. and i.v. Suppressor activity decreased 50-60 days after bone marrow transplantation in i.v. chimeras, while there was no decrease in i.s. chimeras. In vitro suppressor activity was correlated with in vivo activity. Histopathological changes in the liver were examined. A total of 28% of the i.v. chimeras showed severe changes compared with 6% of the i.s. chimeras. The spleen indices of i.v. and i.s. chimeras were compared. Although there was no statistically significant difference between the spleen indices of i.v. chimeras and those of i.s. chimeras, spleen indices of i.v. chimeras tended to be higher than those of i.s. chimeras. These results show that suppressor cells in i.s. chimeras appear to inhibit graft-versus-host reactions more efficiently. Furthermore, an adoptive transfer assay showed that suppressor cells detected in i.s. chimeras were effective in vivo. We therefore suggest that suppressor cells detected in vitro correlate with in vivo activity and may play some role in the induction and maintenance of transplantation tolerance.
在受辐照的C3H/He受体小鼠中，经脾内注射（i.s.）BALB/c骨髓细胞诱导的存活率高于静脉注射（i.v.）。共培养实验揭示，经脾内和静脉注射的小鼠脾脏中存在同种异体抗原特异性和非特异性抑制细胞。静脉注射嵌合体小鼠在骨髓移植后50 - 60天抑制活性下降，而脾内注射嵌合体小鼠则没有下降。体外抑制活性与体内活性相关。对肝脏的组织病理学变化进行了检查。静脉注射嵌合体小鼠中有28%出现严重变化，相比之下，脾内注射嵌合体小鼠为6%。对静脉注射和脾内注射嵌合体小鼠的脾脏指数进行了比较。尽管静脉注射嵌合体小鼠和脾内注射嵌合体小鼠的脾脏指数之间没有统计学上的显著差异，但静脉注射嵌合体小鼠的脾脏指数往往高于脾内注射嵌合体小鼠。这些结果表明，脾内注射嵌合体小鼠中的抑制细胞似乎能更有效地抑制移植物抗宿主反应。此外，过继转移试验表明，在脾内注射嵌合体小鼠中检测到的抑制细胞在体内是有效的。因此，我们认为在体外检测到的抑制细胞与体内活性相关，并且可能在移植耐受的诱导和维持中发挥一定作用。
