Imamura M
Jpn J Exp Med. 1986 Oct;56(5):221-8.
Bone marrow cells from BALB/c mice were transplanted into lethally X-irradiated C3H/He mice, either intravenously (i.v.) or intrasplenically (i.s.). Chimeras created by intrasplenic transplantation (designated i.s. chimeras) showed a higher survival rate than chimeras created by intravenous transplantation (designated i.v. chimeras). During the course of the study on the mechanisms which caused such a difference, suppressor cells were detected in the spleen of i.s. chimeras by means of MLR co-culture assay and CTL assay. Although these cells were also detected in i.v. chimeras, time course study of the suppressor activity against donor anti-host MLR in i.v. and i.s. chimeras showed some difference. Namely, the suppressor activity in the i.s. chimeras was more constant than that in the i.v. chimeras. This difference may induce a beneficial effect of i.s. bone marrow transplantation.
将BALB/c小鼠的骨髓细胞通过静脉内（i.v.）或脾内（i.s.）移植到经致死剂量X射线照射的C3H/He小鼠体内。通过脾内移植产生的嵌合体（称为i.s.嵌合体）比通过静脉移植产生的嵌合体（称为i.v.嵌合体）表现出更高的存活率。在研究导致这种差异的机制过程中，通过混合淋巴细胞反应（MLR）共培养测定法和细胞毒性T淋巴细胞（CTL）测定法在i.s.嵌合体的脾脏中检测到了抑制细胞。尽管在i.v.嵌合体中也检测到了这些细胞，但对i.v.和i.s.嵌合体中针对供体抗宿主MLR的抑制活性的时间进程研究显示出一些差异。也就是说，i.s.嵌合体中的抑制活性比i.v.嵌合体中的更稳定。这种差异可能会诱导i.s.骨髓移植产生有益效果。
