a Departments of Physics and Astronomy.
d Trev and Joyce Deeley Research Centre, BC Cancer Victoria Centre, Victoria, Canada.
Radiat Res. 2018 May;189(5):497-504. doi: 10.1667/RR15003.1. Epub 2018 Feb 23.
Radiation therapy plays a crucial role in the management of breast cancer. However, current standards of care have yet to accommodate patient-specific radiation sensitivity. Raman spectroscopy is promising for applications in radiobiological studies and as a technique for personalized radiation oncology, since it can detect spectral changes in irradiated tissues. In this study, we used established Raman spectroscopic approaches to investigate the biochemical nature and temporal evolution of spectral changes in human breast adenocarcinoma xenografts after in vivo irradiation. Spectral alterations related to cell cycle variations with radiation dose were identified for tumors treated using a range of single-fraction ionizing radiation doses. Additional dose-dependent spectral changes linked to specific proteins, nucleic acids and lipids were also identified in irradiated tumors with a clear temporal evolution of the expression of these signatures. This study reveals distinct shifts in Raman spectra after in vivo irradiation of human breast adenocarcinoma xenografts, emphasizing the significance of Raman spectroscopy for assessing tumor response during radiation therapy.
放射治疗在乳腺癌的治疗中起着至关重要的作用。然而，目前的治疗标准尚未考虑到患者的辐射敏感性。拉曼光谱在放射生物学研究和个性化放射肿瘤学方面具有广阔的应用前景，因为它可以检测照射组织的光谱变化。在这项研究中，我们使用已建立的拉曼光谱方法研究了人乳腺癌异种移植瘤在体内照射后的生化性质和光谱变化的时间演变。我们鉴定了与细胞周期变化相关的光谱改变，这些改变与使用一系列单次电离辐射剂量治疗的肿瘤有关。在照射的肿瘤中，我们还发现了与特定蛋白质、核酸和脂质相关的额外的剂量依赖性光谱改变，这些特征的表达具有明确的时间演变。这项研究揭示了人乳腺癌异种移植瘤体内照射后的拉曼光谱明显改变，强调了拉曼光谱在评估放射治疗期间肿瘤反应方面的重要性。
