Department of Physics, I.K. Barber School of Arts and Sciences, University of British Columbia - Okanagan, Kelowna, V1 V 1 V7, Canada.
Analyst. 2018 Aug 6;143(16):3850-3858. doi: 10.1039/c8an00345a.
External beam radiotherapy is a common form of treatment for breast cancer. Among patients and across different breast cancer subtypes, the response to radiation is heterogeneous. Radiation-induced biochemical changes were examined by Raman spectroscopy using cell lines that represent a spectrum of human breast cancer. Principal component analysis (PCA) and partial least squares discriminant analysis (PLSDA) revealed unique Raman spectral features in the HER2 and Ki67 subtype. The changes in Raman spectral profiles to different doses of radiation (0-50 Gy) included variations in the levels of proteins, lipids, nucleic acids and glycogen. Importantly, the differences in radiation-induced changes on the normal breast epithelial cell line MCF10A could be discriminated within and across the various breast tumor cell lines. These results demonstrate a novel approach to uncover differences between breast cancer cell subtypes and surrounding normal tissues by their biochemical variations in response to radiation.
体外放射治疗是乳腺癌的一种常见治疗形式。在患者和不同的乳腺癌亚型中，对辐射的反应是不均匀的。使用代表一系列人类乳腺癌的细胞系，通过拉曼光谱检查辐射引起的生化变化。主成分分析（PCA）和偏最小二乘判别分析（PLSDA）在 HER2 和 Ki67 亚型中显示出独特的拉曼光谱特征。拉曼光谱谱图对不同剂量辐射（0-50Gy）的变化包括蛋白质、脂质、核酸和糖原水平的变化。重要的是，可以在不同的乳腺癌细胞系内和之间区分正常乳腺上皮细胞系 MCF10A 的辐射诱导变化的差异。这些结果表明，通过辐射反应的生化变化来揭示乳腺癌细胞亚型与周围正常组织之间差异的一种新方法。
