Li Jinxin, Xue Bin, Cheng Xiaoyu, Hu Jiahuan, Hu Jinsheng, Tian Jianghai, Li Fanchi, Yu Xiaohua, Li Bing
School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, P.R. China.
National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu, P.R. China.
J Econ Entomol. 2018 Apr 2;111(2):879-884. doi: 10.1093/jee/toy002.
Silkworm, Bombyx mori (L.; Lepidoptera: Bombycidae), is an economically important insect, which is sensitive to the environment and susceptible to oxidative damages at high temperature. Low concentrations of TiO2 NPs (titanium dioxide nanoparticles) can scavenge reactive oxygen species (ROS) produced by oxidative damages in vivo. To explore whether TiO2 NPs could alleviate oxidative damages of high temperature, the effects of TiO2 NPs treatment on silkworm growth, the levels of ROS and H2O2, as well as the transcription level of antioxidant-related genes were studied at 30°C. These results showed that TiO2 NPs treatment increased silkworm body weight by 6.0% and reduced the occurrence of irregular cocoon at 30°C. TiO2 NPs treatment at 30°C decreased ROS levels in fat body and increased expression of Hsp70, SOD by 5.70-fold at 48 h, TPx by 1.61-fold, CAT by 1.81-fold. These results indicated that TiO2 NPs treatment at 30°C could promote the expression of antioxidant genes and reduce oxidative stress and provide a new method to alleviate high-temperature induced oxidative stress to silkworm.
家蚕，学名Bombyx mori（L.；鳞翅目：蚕蛾科），是一种具有重要经济价值的昆虫，其对环境敏感，在高温下易受氧化损伤。低浓度的二氧化钛纳米颗粒（TiO2 NPs）能够清除体内氧化损伤产生的活性氧（ROS）。为探究TiO2 NPs是否能减轻高温引起的氧化损伤，在30°C条件下研究了TiO2 NPs处理对家蚕生长、ROS和H2O2水平以及抗氧化相关基因转录水平的影响。结果表明，在30°C时，TiO2 NPs处理使家蚕体重增加了6.0%，并减少了不规则蚕茧的出现。在30°C下进行TiO2 NPs处理，48小时时脂肪体中的ROS水平降低，热休克蛋白70（Hsp70）、超氧化物歧化酶（SOD）表达量增加5.70倍，硫氧还蛋白（TPx）增加1.61倍，过氧化氢酶（CAT）增加1.81倍。这些结果表明，30°C下TiO2 NPs处理可促进抗氧化基因的表达，减轻氧化应激，为缓解高温诱导的家蚕氧化应激提供了一种新方法。
