Lu Qisheng, Gong Yulong, Xi Longwei, Liu Yulong, Xu Wenjie, Liu Haokun, Jin Junyan, Zhang Zhimin, Yang Yunxia, Zhu Xiaoming, Xie Shouqi, Han Dong
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Antioxidants (Basel). 2022 May 17;11(5):980. doi: 10.3390/antiox11050980.
Caloric restriction is known to suppress oxidative stress in organ systems. However, whether caloric/feed restriction alleviates chronic thermal stress in aquatic animals remains unknown. Here, we set up three feeding rations: 3% BW (3% body weight/day), 2.5% BW (restricted feeding, 2.5% body weight/day) and 2% BW (high restricted feeding, 2% body weight/day), to investigate the effects and mechanism of feed restriction on improving chronic heat-induced (27 to 31 °C) liver peroxidation and damages in channel catfish (). The results showed that, compared to 3% BW, both 2.5% BW and 2% BW significantly reduced the liver expressions of hsc70, hsp70 and hsp90, but only 2.5% BW did not reduce the growth performance of channel catfish. The 2.5% BW and 2% BW also reduced the lipid deposition (TG) and improved the antioxidant capacity (CAT, SOD, GSH and T-AOC) in the liver of channel catfish. The heat-induced stress response (plasma glucose, cortisol and NO) and peroxidation (ROS and MDA) were also suppressed by either 2.5% BW or 2% BW. Moreover, 2.5% BW or 2% BW overtly alleviated liver inflammation and damages by reducing endoplasmic reticulum (ER) stress (BIP and Calnexin) and cell apoptosis (BAX, Caspase 3 and Caspase 9) in the liver of channel catfish. In conclusion, 2.5% body weight/day is recommended to improve the antioxidant capacity and liver health of channel catfish during the summer season, as it alleviates liver peroxidation and damages via suppressing lipid accumulation under chronic thermal stress.
热量限制已知可抑制器官系统中的氧化应激。然而，热量/饲料限制是否能减轻水生动物的慢性热应激仍不清楚。在此，我们设置了三种投喂量：3%体重（3%体重/天）、2.5%体重（限制投喂，2.5%体重/天）和2%体重（高度限制投喂，2%体重/天），以研究饲料限制对改善斑点叉尾鮰慢性热诱导（27至31°C）肝脏过氧化和损伤的影响及机制。结果表明，与3%体重组相比，2.5%体重组和2%体重组均显著降低了肝脏中hsc70、hsp70和hsp90的表达，但只有2.5%体重组未降低斑点叉尾鮰的生长性能。2.5%体重组和2%体重组还减少了斑点叉尾鮰肝脏中的脂质沉积（TG），并提高了抗氧化能力（CAT、SOD、GSH和T-AOC）。2.5%体重组或2%体重组也抑制了热诱导的应激反应（血浆葡萄糖、皮质醇和NO）和过氧化（ROS和MDA）。此外，2.5%体重组或2%体重组通过降低斑点叉尾鮰肝脏中的内质网（ER）应激（BIP和钙连接蛋白）和细胞凋亡（BAX、半胱天冬酶3和半胱天冬酶9），明显减轻了肝脏炎症和损伤。总之，建议夏季每天投喂2.5%体重，以提高斑点叉尾鮰的抗氧化能力和肝脏健康，因为它通过抑制慢性热应激下的脂质积累来减轻肝脏过氧化和损伤。
