从感染细胞培养上清液中简单快速浓缩 Zika 病毒颗粒的方法。
Method for simple and rapid concentration of Zika virus particles from infected cell-culture supernatants.
机构信息
Institut Louis Malardé, PO Box 30, 98713 Papeete, French Polynesia; Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Méditerranée Infection, Marseille, France.
Institut Louis Malardé, PO Box 30, 98713 Papeete, French Polynesia; Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Méditerranée Infection, Marseille, France.
出版信息
J Virol Methods. 2018 May;255:82-83. doi: 10.1016/j.jviromet.2018.02.014. Epub 2018 Feb 21.
Experimental studies on Zika virus (ZIKV) may require improvement of infectious titers in viral stocks obtained by cell culture amplification. The use of centrifugal filter devices to increase infectious titers of ZIKV from cell-culture supernatants is highlighted here. A mean gain of 2.33 ± 0.12 log DICT/mL was easily and rapidly obtained with this process. This efficient method of ultrafiltration may be applied to other viruses and be useful in various experimental studies requiring high viral titers.
实验研究寨卡病毒（ZIKV）可能需要提高细胞培养扩增获得的病毒滴度。本文强调了使用离心过滤装置提高细胞培养上清液中寨卡病毒的感染滴度。该过程可轻松快速地获得平均 2.33±0.12 log DICT/mL 的增益。这种有效的超滤方法可应用于其他病毒，并且在需要高病毒滴度的各种实验研究中非常有用。
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