Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, 04103 Leipzig, Germany.
Sartorius Lab Instruments, 37079 Göttingen, Germany.
J Virol Methods. 2022 Dec;310:114628. doi: 10.1016/j.jviromet.2022.114628. Epub 2022 Oct 6.
Enrichment of viral infectious titers following its propagation by cell culture is desirable for various experimental studies. The performance of an ultrafiltration (UF) process to concentrate infectious titers of non-enveloped Canine parvovirus 2 (CPV-2) and enveloped Feline coronavirus (FCoV) obtained from cell culture supernatants was evaluated in this study, and compared with ultracentrifugation (UC) process. A mean gain of > 1.0 log TCID/mL was obtained for CPV-2 with UF, which was comparable with the gain obtained by UC. On the other hand, the gain was lower (0.7-1.0 log TCID/mL) for FCoV with UF in contrast to UC (> 2.0 log TCID/mL). However, the lower retentate volume following UC (∼120 fold) compared to that following UF (∼10 fold) for either of the viruses suggests a trend of increased infectious titer retention in UF concentrates relative to UC concentrates. The simplistic UF process evaluated here thus has the potential for use in applications requiring increased infectious titers of CPV-2 and FCoV.
在细胞培养中增殖病毒后，提高病毒感染滴度对于各种实验研究都是非常理想的。本研究评估了超滤（UF）工艺浓缩来自细胞培养上清液的非包膜犬细小病毒 2（CPV-2）和包膜猫冠状病毒（FCoV）的传染性滴度的性能，并与超速离心（UC）工艺进行了比较。对于 CPV-2，UF 的平均增益> 1.0 log TCID/mL，与 UC 获得的增益相当。另一方面，对于 UF 来说，FCoV 的增益较低（0.7-1.0 log TCID/mL），而对于 UC 来说，则较高（> 2.0 log TCID/mL）。然而，与 UC 相比，UF 对两种病毒的浓缩物的保留体积较小（分别约为 120 倍和约 10 倍），这表明 UF 浓缩物中病毒感染滴度的保留趋势相对 UC 浓缩物有所增加。因此，这里评估的简单 UF 工艺具有提高 CPV-2 和 FCoV 的感染滴度的应用潜力。
