Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.
Microb Pathog. 2018 Apr;117:304-309. doi: 10.1016/j.micpath.2018.02.031. Epub 2018 Feb 20.
The pathogenic bacteria of Corynebacterium pseudotuberculosis caused a chronic contagious infectious disease of the caseous lymphadenitis or pseudotuberculosis. Globally, isolates obtained from different injuries that affect sheep and goats, have been identified by fully or partially gene sequencing. However, in Mexico there is no complete study to identify by molecular and phylogenetic techniques the circulating isolates as well as its virulence factors. Therefore, in the present study we reported the identification of 57 isolates of C. pseudotuberculosis by bacteriological tests and the amplification of 16S rRNA, rpoB and pld genes, as well as, genes involved in virulence and pathogenicity: Fag A, Fag B, Fag C, Fag D and hsp60. Phylogenetic analysis was performed based on the partial sequence of the rpoB gene. Genes involved in virulence and pathogenicity were identified in the 98.2% of the isolates. Regarding the phylogenetic analysis, were identified the species and subspecies to which they belong of all the tested isolates. The phenotypic and genotypic characterization will allow to establish preventive and prophylactic measures aimed to the creation of effective immunogens against Corynebacterium pseudotuberculosis.
类白喉棒状杆菌的病原菌引起干酪性淋巴结炎或类结核病的慢性传染性疾病。在全球范围内，已通过完全或部分基因测序鉴定出从不同损伤中获得的、影响绵羊和山羊的分离株。然而，在墨西哥，没有通过分子和系统发育技术对循环分离株及其毒力因子进行完整的研究。因此，在本研究中，我们通过细菌学试验和 16S rRNA、rpoB 和 pld 基因的扩增，以及参与毒力和致病性的基因：Fag A、Fag B、Fag C、Fag D 和 hsp60，报告了 57 株类白喉棒状杆菌的鉴定。基于 rpoB 基因的部分序列进行了系统发育分析。在 98.2%的分离株中鉴定出了与毒力和致病性相关的基因。关于系统发育分析，对所有测试的分离株进行了鉴定，以确定它们所属的种和亚种。表型和基因型特征将有助于制定针对类白喉棒状杆菌的预防性和预防性措施，以创建有效的免疫原。
