Research and Advanced Studies in Animal Health Center, Faculty of Veterinary Medicine and Zootechnics, Autonomy University of the State of Mexico, Km 15.5 Toluca Pan-American Highway Atlacomulco, Toluca C.P. 50200, State of Mexico, Mexico.
Sor Juana Inés de la Cruz School, Autonomy University of the State of Mexico- AMECAMECA, Amecameca de Juarez C.P. 56900, State of Mexico, Mexico.
Int J Mol Sci. 2023 Dec 23;25(1):270. doi: 10.3390/ijms25010270.
Mapping B and T cell epitopes constitutes an important action for peptide vaccine design. PLD and CP40 virulence factors of biovar , a causal agent of Caseous Lymphadenitis, have been evaluated in a murine model as good candidates for vaccine development. Therefore, the goal of this work was to in silico analyze B and T cell epitopes of the PLD and CP40 proteins of a Mexican isolate of . The Immune Epitope Data Base and Resource website was employed to predict the linear and conformational B-cell, T CD4+, and T CD8+ epitopes of PLD and CP40 proteins of Mexican strain 2J-L. Fifty B cell epitopes for PLD 2J-L and forty-seven for CP40 2J-L were estimated. In addition, T CD4+ and CD8+ cell epitopes were predicted for PLD 2J-L (MHC I:16 epitopes, MHC II:10 epitopes) and CP40 2J-L (MHC I: 15 epitopes, MHC II: 13 epitopes). This study provides epitopes, paying particular attention to sequences selected by different predictor programs and overlap sequences as B and T cell epitopes. PLD 2J-L and CP40 2J-L protein epitopes may aid in the design of a promising peptide-based vaccine against Caseous Lymphadenitis in Mexico.
对 B 细胞和 T 细胞表位进行定位是设计肽疫苗的重要步骤。副结核分枝杆菌生物型的 PLD 和 CP40 毒力因子被评估为牛分枝杆菌性淋巴结炎疫苗开发的良好候选物，已在小鼠模型中进行了评估。因此，本工作的目的是通过计算机分析来自墨西哥分离株 2J-L 的 PLD 和 CP40 蛋白的 B 和 T 细胞表位。使用免疫表位数据库和资源网站预测了墨西哥株 2J-L 的 PLD 和 CP40 蛋白的线性和构象 B 细胞、T CD4+和 T CD8+表位。估计了 2J-L 的 50 个 PLD B 细胞表位和 47 个 CP40 B 细胞表位。此外，还预测了 PLD 2J-L（MHC I:16 个表位，MHC II:10 个表位）和 CP40 2J-L（MHC I:15 个表位，MHC II:13 个表位）的 T CD4+和 CD8+细胞表位。本研究提供了表位，特别注意了不同预测程序选择的序列和作为 B 和 T 细胞表位的重叠序列。PLD 2J-L 和 CP40 2J-L 蛋白表位可能有助于设计针对墨西哥干酪样淋巴结炎的有前途的基于肽的疫苗。
