采用溶菌酶预处理后再用Trizol法从枯草芽孢杆菌中提取高质量RNA。
Extraction of high-quality RNA from Bacillus subtilis with a lysozyme pre-treatment followed by the Trizol method.
作者信息
Villa-Rodríguez Eber, Ibarra-Gámez Cuauhtemoc, de Los Santos-Villalobos Sergio
机构信息
Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, C.P.85000, Col. Centro, Ciudad Obregón, Sonora, Mexico.
CONACYT- Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, C.P.85000, Col. Centro, Ciudad Obregón, Sonora, Mexico..
出版信息
J Microbiol Methods. 2018 Apr;147:14-16. doi: 10.1016/j.mimet.2018.02.011. Epub 2018 Feb 21.
A suitable technique (lysozyme combined with Trizol reagent) was developed to improve the RNA yield, purity and integrity from Bacillus subtilis, under different bacterial growth stages. The obtained RNA was intact, having the required characteristics for downstream applications.
开发了一种合适的技术（溶菌酶与Trizol试剂结合），以提高不同细菌生长阶段下枯草芽孢杆菌的RNA产量、纯度和完整性。所获得的RNA完整无损，具有下游应用所需的特性。
Zotero 插件