Cai Xinghua, Sun Huijie, Yan Bing, Bai Huashan, Zhou Xing, Shen Peihong, Jiang Chengjian
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, China.
Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai, China.
Front Microbiol. 2023 Jul 17;14:1193352. doi: 10.3389/fmicb.2023.1193352. eCollection 2023.
Extremely salt-tolerant microorganisms play an important role in the development of functional metabolites or drug molecules.
In this work, the salt stress perception and metabolic regulation network of a marine probiotic GXDK6 were investigated using integrative omics technology.
Results indicated that GXDK6 could accept the salt stress signals from signal transduction proteins (e.g., phosphorelay intermediate protein YPD1), thereby contributing to regulating the differential expression of its relevant genes (e.g., , ) and proteins (e.g., catalase, superoxide dismutase) in response to salt stress, and increasing the salt-tolerant viability of GXDK6. Omics data also suggested that the transcription (e.g., ), translation (e.g., ), and protein synthesis and processing (e.g., inner membrane protein OXA1) of upregulated RNAs may contribute to increasing the salt-tolerant survivability of GXDK6 by improving protein transport activity (e.g., Small nuclear ribonucleoprotein Sm D2), anti-apoptotic ability (e.g., 54S ribosomal protein L1), and antioxidant activity (e.g., superoxide dismutase). Moreover, up to 65.9% of the differentially expressed genes/proteins could stimulate GXDK6 to biosynthesize many salt tolerant-related metabolites (e.g., β-alanine, D-mannose) and drug molecules (e.g., deoxyspergualin, calcitriol), and were involved in the metabolic regulation of GXDK6 under high NaCl stress.
This study provided new insights into the exploration of novel functional products and/or drugs from extremely salt-tolerant microorganisms.Graphical Abstract.
极端耐盐微生物在功能性代谢产物或药物分子的开发中发挥着重要作用。
在本研究中，利用整合组学技术研究了海洋益生菌GXDK6的盐胁迫感知和代谢调控网络。
结果表明，GXDK6可以接受来自信号转导蛋白（如磷酸转移中间蛋白YPD1）的盐胁迫信号，从而有助于调节其相关基因（如 ， ）和蛋白质（如过氧化氢酶、超氧化物歧化酶）在盐胁迫下的差异表达，并提高GXDK6的耐盐生存能力。组学数据还表明，上调RNA的转录（如 ）、翻译（如 ）以及蛋白质合成和加工（如内膜蛋白OXA1）可能通过提高蛋白质转运活性（如小核核糖核蛋白Sm D2）、抗凋亡能力（如54S核糖体蛋白L1）和抗氧化活性（如超氧化物歧化酶）来提高GXDK6的耐盐生存能力。此外，高达65.9%的差异表达基因/蛋白质可以刺激GXDK6生物合成许多耐盐相关代谢产物（如β-丙氨酸、D-甘露糖）和药物分子（如去氧精胍菌素、骨化三醇），并参与高NaCl胁迫下GXDK6的代谢调控。
本研究为从极端耐盐微生物中探索新型功能产品和/或药物提供了新的见解。图形摘要。
