School of Pharmacy, Nantong University, Nantong 226001, PR China.
Department of Analysis and Testing Center, Nantong University, Nantong 226001, PR China.
Fitoterapia. 2018 Jun;127:207-211. doi: 10.1016/j.fitote.2018.02.012. Epub 2018 Feb 20.
As an attempt to utilize of rare earth elements as a novel method to activate the silent genes in fungus, the marine-derived fungus Penicillium citrinum was cultured under ordinary laboratory fermentation conditions in the presence of scandium chloride (ScCl, 50 μM), and chemical investigation led to the isolation and characterization of three new peptide derivatives (1-3), along with four known pyrrolidine alkaloids (4-7). Those structures were elucidated by spectroscopic data interpretation, as well as chemical reactions. Comparative metabolic profiling of the culture extracts (with/without scandium chloride) indicated that compounds 1-3 scarcely detected in the absence of ScCl. In addition, the antibacterial and cytotoxic activities of all isolated products were evaluated.
作为一种尝试，利用稀土元素作为一种新的方法来激活真菌中的沉默基因，在氯化钪（ScCl，50μM）的存在下，对海洋来源的真菌青霉（Penicillium citrinum）进行了普通实验室发酵条件下的培养。化学研究导致分离和表征了三个新的肽衍生物（1-3），以及四个已知的吡咯烷生物碱（4-7）。这些结构是通过光谱数据分析以及化学反应来阐明的。培养提取物（有/没有氯化钪）的比较代谢谱分析表明，在没有 ScCl 的情况下，化合物 1-3 几乎检测不到。此外，还评估了所有分离产物的抗菌和细胞毒性活性。
