Institute of Medical Sciences, Tzu-Chi University, Hualien 970, Taiwan.
Department of Laboratory Medicine and Biotechnology, Tzu-Chi University, Hualien 970, Taiwan; Department of Laboratory Medicine, Buddhist Tzu-Chi General Hospital, Hualien, Taiwan.
J Photochem Photobiol B. 2018 Mar;180:235-242. doi: 10.1016/j.jphotobiol.2018.02.003. Epub 2018 Feb 6.
Photodynamic inactivation (PDI) is a non-invasive and safe therapeutic method for microbial infections. Bacterial antibiotic resistance is caused by antibiotics abuse. Drug-resistant Acinetobacter spp. is a serious problem in hospitals around the world. These pathogens from nosocomial infections have high mortality rates in frailer people, and Acinetobacter spp. is commonly found in immunocompromised patients. Visible light is safer than ultraviolet light (UV) for PDI of nosocomial pathogens with mammalian cells. Zinc oxide nanoparticles (ZnO-NPs) were used in this study as an antimicrobial agent and a photosensitizer. ZnO is recognized as safe and has extensive usage in food additives, medical and cosmetic products. In this study, we used 0.125 mg/ml ZnO-NPs combined with 10.8 J/cm blue light (BL) on Acinetobacter baumannii (A. baumannii) that could significantly reduce microbial survival. However, individual exposure to ZnO-NPs does not affect the viability of A. baumannii. BL irradiation could trigger the antimicrobial ability of ZnO nanoparticles on A. baumannii. The mechanism of photocatalytic ZnO-NPs treatment for sterilization occurs through bacterial membrane disruptions. Otherwise, the photocatalytic ZnO-NPs treatment showed high microbial eradication in nosocomial pathogens, including colistin-resistant and imipenem-resistant A. baumannii and Klebsiella pneumoniae. Based on our results, the photocatalytic ZnO-NPs treatment could support hygiene control and clinical therapies without antibiotics to nosocomial bacterial infections.
光动力灭活（PDI）是一种非侵入性和安全的治疗微生物感染的方法。抗生素的滥用导致了细菌的抗生素耐药性。耐抗生素不动杆菌属是世界各地医院的一个严重问题。这些来自医院感染的病原体在身体较弱的人群中死亡率较高，而免疫功能低下的患者通常会感染不动杆菌属。可见光比紫外线（UV）更安全，可用于对哺乳动物细胞进行 PDI 治疗医院病原体。在这项研究中，氧化锌纳米粒子（ZnO-NPs）被用作抗菌剂和光敏剂。氧化锌被认为是安全的，并且在食品添加剂、医疗和化妆品产品中有广泛的应用。在这项研究中，我们使用 0.125mg/ml 的 ZnO-NPs 结合 10.8J/cm 的蓝光（BL）照射鲍曼不动杆菌（A.baumannii），可以显著降低微生物的存活率。然而，单独暴露于 ZnO-NPs 不会影响 A.baumannii 的活力。BL 照射可以触发 ZnO 纳米粒子对 A.baumannii 的抗菌能力。光催化 ZnO-NPs 处理杀菌的机制是通过细菌膜破裂。此外，光催化 ZnO-NPs 处理在包括多粘菌素耐药和亚胺培南耐药鲍曼不动杆菌和肺炎克雷伯菌在内的医院病原体中表现出很高的微生物清除率。基于我们的结果，光催化 ZnO-NPs 处理可以支持无抗生素的医院细菌感染的卫生控制和临床治疗。
