"Ilie Murgulescu" Institute of Physical Chemistry, Romanian Academy, Spl. Independentei 202, 060021 Bucharest, Romania.
Department of Microbiology, Faculty of Biology, University of Bucharest, Research Institute of University of Bucharest, ICUB, Ale. Portocalilor 1-3, 60101 Bucharest, Romania.
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:438-450. doi: 10.1016/j.msec.2018.12.063. Epub 2018 Dec 19.
ZnO materials with spherical morphology, core-shell and solid, disperse or interconnected, were obtained by a completely green synthesis via a carbohydrate-template route. Morphology, structure and optical properties, as well as antimicrobial potential and cytocompatibility were investigated. The antimicrobial efficiency of the obtained materials was screened against a large spectrum of reference and clinical microbial strains, both susceptible and exhibiting resistance phenotypes of clinical and epidemiological interest, in planktonic and biofilm state. Their biocidal activity is strongly dependent of material's characteristics and target microorganism. One of the most valuable findings of our study is the good antibiofilm activity of the obtained nanostructures, which in some cases was superior to that noted against planktonic cells, despite the well-known high tolerance of biofilm-embedded cells to different stressor agents. Another important finding is the excellent efficiency against three Gram-negative, respectively Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae and two Gram-positive species, i.e. Staphylococcus aureus and Enteroccus faecium included in the ESKAPE list of the most dangerous resistant pathogens, requiring global surveillance and urgent need for the development of novel antimicrobial agents. Our study offers the first insight regarding the high therapeutic potential of ZnO nanoparticles against the fearful nosocomial pathogen A. baumannii. The cytocompatibility of the developed materials in terms of cell morphology, viability and proliferation, revealed a comparable dose-dependent cellular response, at the active antimicrobial concentrations, only a low effect on cell viability is evidenced. Overall, our data demonstrated the potential of the materials for antimicrobial applications and also that their biotoxicity can be modulated directly through their morpho-structural characteristics.
通过碳水化合物模板路线的完全绿色合成，获得了具有球形形态、核壳和实心、分散或相互连接的 ZnO 材料。研究了其形貌、结构和光学性能，以及抗菌潜力和细胞相容性。通过筛选获得的材料对大光谱的参考和临床微生物菌株的抗菌效率，包括敏感和表现出临床和流行病学意义的耐药表型的浮游和生物膜状态，进行了筛选。它们的杀菌活性强烈依赖于材料的特性和目标微生物。我们研究的最有价值的发现之一是，所获得的纳米结构具有良好的抗生物膜活性，在某些情况下，其活性优于对浮游细胞的活性，尽管众所周知，生物膜嵌入的细胞对不同的应激剂具有很高的耐受性。另一个重要的发现是，对三种革兰氏阴性菌（分别为铜绿假单胞菌、鲍曼不动杆菌和肺炎克雷伯菌）和两种革兰氏阳性菌（即金黄色葡萄球菌和屎肠球菌）的高效性，这些菌均被列入了最危险的耐药病原体 ESKAPE 名单中，需要进行全球监测，并迫切需要开发新的抗菌剂。我们的研究首次揭示了 ZnO 纳米粒子对可怕的医院病原体鲍曼不动杆菌的高治疗潜力。从细胞形态、活力和增殖的角度来看，开发材料的细胞相容性揭示了一种在活性抗菌浓度下与剂量相关的细胞反应，仅在细胞活力方面显示出较低的影响。总体而言，我们的数据证明了这些材料在抗菌应用方面的潜力，同时也表明它们的生物毒性可以通过其形态结构特征直接调节。
