Goto Yuta, Okamoto Atsushi, Shibukawa Atsushi, Ogawa Kazuhisa, Tomita Akihisa
Opt Express. 2018 Feb 19;26(4):3779-3790. doi: 10.1364/OE.26.003779.
We propose a virtual phase conjugation (VPC) based optical tomography (VPC-OT) for realizing single-shot optical tomographic imaging systems. Using a computer-based numerical beam propagation, the VPC combines pre-modulation and post-demodulation of the probe beam's wavefront, which provides an optical sectioning capability for resolving the depth coordinates. In VPC-OT, the physical optical microscope system and VPC are coupled using digital holography. Therefore, in contrast to conventional optical tomographic imaging (OTI) systems, this method does not require additional elements such as low-coherence light sources or confocal pinholes. It is challenging to obtain single-shot three-dimensional (3D) tomographic images using a conventional OTI system; however, this can be achieved using VPC-OT, which employs both digital holography and computer based numerical beam propagation. In addition, taking into account that VPC-OT is based on a complex amplitude detection using digital holography, this method allows us to simultaneously obtain quantitative phase contrast images. Using an objective lens with a numerical aperture (NA) of 0.8, we demonstrate a single-shot 3D imaging of frog blood cells with a depth resolution of 0.94 μm.
我们提出了一种基于虚拟相位共轭（VPC）的光学层析成像（VPC-OT）技术，以实现单次光学层析成像系统。通过基于计算机的数值光束传播，VPC将探测光束波前的预调制和后解调相结合，从而提供了用于解析深度坐标的光学切片能力。在VPC-OT中，物理光学显微镜系统和VPC通过数字全息术进行耦合。因此，与传统光学层析成像（OTI）系统相比，该方法不需要诸如低相干光源或共焦针孔等额外元件。使用传统OTI系统获取单次三维（3D）层析图像具有挑战性；然而，这可以通过采用数字全息术和基于计算机的数值光束传播的VPC-OT来实现。此外，考虑到VPC-OT基于使用数字全息术的复振幅检测，该方法使我们能够同时获得定量相衬图像。使用数值孔径（NA）为0.8的物镜，我们展示了对青蛙血细胞的单次3D成像，深度分辨率为0.94μm。
